Fig. 3

Effects of PP7 on morphological changes, heart beat rate, survival rate, ISVs development and SIVs formation in zebrafish embryos. a The morphological features of zebrafish embryos observed and captured after treating embryos with PP7 for 24, 48 and 72 h. Scale bars represent 200 μM. b The average heart beating rates per minute of PP7 treated embryos were recorded at 72 h. c Survival rates of zebrafish embryos after treatment with PP7 for 24, 48 and 72 h. Lateral view of transgenic Tg(fli1:EGFP) zebrafish showing ISV (d) and SIV (g) growth after treatment for 24 h. Scale bars represent 500 μm (d) or 150 μm (g). Arrow showed the characteristic pattern of SIV. e and f was statistical analysis of number and length of ISVs of d, respectively. h, i was statistical analysis of number and length of SIVs of g, respectively. n = 10/group, *P < 0.05, **P < 0.01 as compared with control