Fig. 5

Effect of SF on Treg and Th17 cells in the spleen and lymph nodes of CIA mice. a Representative image of CD4⁺CD25⁺Foxp3⁺ cells in spleen after one-month administration. b Representative image of CD4⁺IL-17A⁺ cells in spleen after one-month administration. c Proportion of Treg and Th17 cells and ratio of Treg/Th17 in the spleen of each group of mice (n = 5). d Representative image of CD4⁺CD25⁺Foxp3⁺ cells in lymph nodes after 1-month administration. e Representative image of CD4⁺IL-17A⁺ cells in lymph nodes after 1-month administration. f Proportion of Treg and Th17 cells and ratio of Treg/Th17 in the lymph nodes of each group of mice (n = 5). One-way ANOVA and post hoc Dunnett’s test were performed between multiple groups. ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001, compared with Normal group; *p < 0.05, **p < 0.01, ***p < 0.001, compared with CIA vehicle group