Fig. 1

Tanshinone I (TI) attenuated t-BHP-induced cell death in H9c2 cells. a The chemical structure of TI. b, c H9c2 cells were treated with TI or t-BHP for a series of concentrations for 12 h or 10 h respectively, and cell viability was both tested by MTT. Cells were administrated with t-BHP for 10 h after treated with TI for 2 h. d Employed MTT method to detect the viability of the cells. e Employed LDH kit to monitor the LDH level. f–g Cells pretreated with TI (1 μM) for 2 h before co-incubation with t-BHP (150 μM) for 6 h, were then stained with Annexin V-PE /7-AAD and detected by flow cytometry. n = 3. ***p < 0.001 vs. t-BHP group