Table 1 Tentative identification of the chemical constituents of Erdong decoction by UHPLC- MS in the negative mode

1

C₂₅H₂₈O₁₆

[M−H]⁻

493.0986, 463.0880, 301.0355, 259.0246, 244.0359

Neomangiferin

A

2

C₂₁H₂₂O₁₂

[M−H]⁻

303.0524, 285.0406, 177.0184

Spiraeoside

S

3

C₁₉H₁₈O₁₁

[M−H]⁻

331.0460, 301.0355, 285.0410, 271.0248, 259.0247

Mangiferin

A

4

C₂₁H₂₁O₁₁

[M−H]⁻

287.0553, 259.0620, 171.3426, 151.5096, 125.0229

Taxifolin 7-rhamnoside

N

5

C₁₉H₁₈O₁₁

[M−H]⁻

331.0461, 301.0355, 285.0404, 258.0169

Isomangiferin

A

6

C₂₇H₃₂O₁₄

[M−H]⁻

255.0662

Liquiritigenin 7,4'-di-O-glucopyranoside

G

7

C₂₆H₂₈O₁₄

[M−H]⁻

503.1202, 473.1099, 443.0980, 383.0772, 353.0668, 203.0360

Apigenin 6-C-glucoside-8-C-arabinoside

S

8

C₂₆H₂₈O₁₆

[M−H]⁻

300.0277, 271.0249, 243.0286, 178.9976

Quercetin-3-O-sambubioside

N

9

C₂₁H₂₂O₉

[M−H]⁻

255.0661, 153.0180, 135.0074, 119.0487

Neoliquiritin

G

10

C₂₇H₃₀O₁₄

[M−H]⁻

541.9865, 506.0078, 479.0923, 255.0665

5-Hydroxy-2-(4-hydroxyphenyl)-4-oxo-4H-chromen-7-yl 2-O-(6-deoxy-α-l-mannopyranosyl)-β-d-glucopyranoside

G

11

C₂₁H₂₂O₉

[M−H]⁻

255.0661, 153.0180, 135.0074, 119.0487

Liquiritin

G

12

C₂₆H₁₄O₁₂

[M−H]⁻

471.0382, 255.0655, 153.0182, 135.0076, 119.0488

1,1',3,4',5,6',8,8'-Octahydroxy-9H,9'H-2,2'-bixanthene-9,9'-dione

G

13

C₂₆H₂₈O₁₃

[M−H]⁻

487.1227, 457.1145, 427.1230, 367.0822, 337.0720

Isomer of chrysin 6-C-arabinoside-8-C-glucoside

S

14

C₂₆H₃₀O₁₃

[M−H]⁻

417.1192, 255.0661, 153.0180, 135.0074, 119.0486

Liquiritin apioside

G

15

C₃₂H₄₀O₁₈

[M−H]⁻

549.1617, 255.0660, 153.0176, 135.0073, 119.0487

Glucoliquirtin asioside

G

16

C₂₁H₂₀O₁₂

[M−H]⁻

301.0346, 300.0276, 272.0300, 271.0249, 255.0298, 178.9979, 151.0024

Hyperoside

N

17

C₂₇H₃₀O₁₆

[M−H]⁻

300.0277, 271.0251, 255.0297, 178.9977

Rutin

N

18

C₂₁H₂₀O₁₀

[M−H]⁻

341.0667, 311.0565, 283.0613, 269.0455

Baicalein 7-O-β-d-glucoside

S

19

C₂₆H₂₈O₁₃

[M−H]⁻

487.1252, 457.1140, 427.1031, 367.0822, 337.0718

Chrysin 6-C-arabinoside-8-C-glucoside

S

20

C₂₆H₃₀O₁₄

[M−H]⁻

438.8078, 295.0642, 271.0612

Hydroxyliquiritin apioside

G

21

C₂₁H₁₈O₁₃

[M−H]⁻

302.0389, 301.0354, 283.0245, 255.0300, 227.0338, 178.9976, 151.0024

Quercetin-3-O-glucuronide

N

22

C₂₆H₂₈O₁₅

[M−H]⁻

284.0328, 255.0293, 227.0346, 151.0025

Leucoside

N

23

C₂₃H₂₄O₁₃

[M−H]⁻

345.0613, 330.0382, 315.0154

Viscidulin III-6'-O-β-d-glucopyranoside

S

24

C₂₁H₂₀O₁₂

[M−H]⁻

300.0276, 271.0250, 255.0296, 178.9976, 151.0024

Isoquercitrin

N

25

C₂₁H₁₈O₁₂

[M−H]⁻

285.0407, 267.0296, 175.0238

Scutellarin

S

26

C₂₆H₂₈O₁₃

[M−H]⁻

457.1138, 427.1029, 367.0823, 337.0719

Chrysin 6-C-glucoside-8-C-arabinoside

S

27

C₂₆H₂₈O₁₃

[M−H]⁻

457.1140, 427.1028, 367.0822, 337.0720

Isomer of chrysin 6-C-arabinoside-8-C-glucoside

S

28

C₂₇H₂₈O₁₆

[M−H]⁻

431.0992, 269.0456

Trihydroxyflavone-glycoside glucuronide

S

29

C₂₇H₂₈O₁₆

[M−H]⁻

445.0771, 431.0983, 269.0455

Trihydroxyflavone-glycoside glucuronide

S

30

C₂₁H₂₀O₉

[M−H]⁻

295.0613, 267.0663, 251.0709, 223.0758

Chrysin 8-C-β-glucoside

S

31

C₂₃H₂₄O₁₀

[M−H]⁻

255.0661, 153.0181, 135.0073, 119.0487

6'-Acetyliquiritin

G

32

C₂₃H₂₄O₁₃

[M−H]⁻

344.0537, 329.0306, 316.0585

Viscidulin III-2'-O-β-d-glucopyranoside

S

33

C₂₇H₃₀O₁₃

[M−H]⁻

471.1297, 441.1179, 281.0830

5-Hydroxy-7-methoxyflavone 6-C-arabinoside-8-C-glucoside or 7-hydroxy-5-methoxyflavone 6-C-arabinoside-8-C-glucoside

S

34

C₂₂H₂₀O₁₂

[M−H]⁻

299.0563, 284.0327

Isomer of hydroxyl oroxylin A 7-O-glucuronide or hydroxyl wogonoside

S

35

C₄₅H₇₆O₂₀

[M−H]⁻

773.4357, 611.3790, 449.3284

Timosaponin E

A

36

C₅₁H₈₄O₂₅

[M−H]⁻

933.4723, 771.4182, 404.0874

(2α,3β,5α,6β,25R)-2,6-Dihydroxyspirostan-3-yl-β-d-glucopyranosyl-(1 → 2)-[β-d-glucopyranosyl-(1 → 3)]-β-d-glucopyranosyl-(1 → 4)-β-d-galactopyranosid

A

37

C₃₂H₂₆O₁₁

[M−H]⁻

549.1618, 539.2637, 417.1174, 297.0774, 255.0662

{3-(4-Hydroxy-3-methoxyphenyl)-6-[(2R,3R)-3,5,7-trihydroxy-4-oxo-3,4-dihydro-2H-chromen-2-yl]-2,3-dihydro-1,4-benzodioxin-2-yl}methyl benzoate

G

38

C₂₆H₃₀O₁₃

[M−H]⁻

255.0661, 153.0180, 135.0074, 119.0487

Isoliquiritin apioside

G

39

C₅₆H₉₂O₂₉

[M−H]⁻

1065.51123, 933.47205、771.41693, 447.31555

3-O-β-d-xylopyranosyl(1 → 4)-[β-d-glucopyranosyl(1 → 2)]-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25S)-5β-furostane-22-methoxy-3β,26-diol

As

40

C₄₈H₈₂O₁₉

[M−H]⁻

799.4863, 637.4363, 475.3780, 391.2874

20-Glc-Rf

P

41

C₄₅H₇₄O₁₉

[M−H]⁻

755.4233, 593.3687, 553.3922, 364.0068, 319.1408

Timosaponin D

A

42

C₅₁H₈₄O₂₄

[M−H]⁻

933.4645, 917.4766, 771.4186, 609.3615

Alliumoside B

A

43

C₂₃H₂₄O₁₀

[M−H]⁻

255.0660, 153.0180, 135.0073, 119.0488

6'-acetylisoliquiritin

G

44

C₂₁H₂₂O₉

[M−H]⁻

255.0661, 153.0179, 119.0487

Isoliquiritin

G

45

C₂₁H₁₈O₁₁

[M−H]⁻

270.0490, 269.0455, 251.0349, 241.0509, 223.0393

Baicalin

S

46

C₂₆H₃₀O₁₃

[M−H]⁻

255.0659, 153.0182, 135.0073, 119.0490

Licuraside

G

47

C₄₅H₇₆O₂₀

[M−H]⁻

773.4354, 611.3803, 449.3252

Timosaponin E1

A

48

C₂₁H₂₂O₉

[M−H]⁻

255.0662

Neoisoliquiritin

G

49

C₂₃H₂₂O₁₃

[M−H]⁻

329.0667, 314.0435, 299.0198, 271.0250, 255.0291, 227.0344, 175.0237

5,6'-Dihydroxy-6,7-dimethoxyflavone 2'-O-β-d-glucuronide

S

50

C₂₁H₂₀O₁₁

[M−H]-

271.0613, 243.0660

Dihydrobaicalin

S

51

C₄₂H₇₂O₁₄

[M−H+HCOOH]⁻

799.4837, 637.4315, 475.3803, 273.3054

Ginsenoside Rg1

P

52

C₄₈H₈₂O₁₈

[M−H+HCOOH]⁻

945.5428, 783.4907, 637.4326, 475.3786

Ginsenoside Re

P

53

C₃₅H₃₆O₁₅

[M−H]⁻

549.1608, 531.1499, 255.0664, 153.0185, 135.0074, 119.0486

Licorice-glycoside B

G

54

C₃₆H₃₈O₁₆

[M−H]⁻

549.1630, 531.1491, 255.0660, 153.0179, 135.0072, 119.0488

Licorice-glycoside A

G

55

C₂₁H₁₈O₁₁

[M−H]⁻

270.0488, 269.0455, 249.0541, 241.0501, 225.0548

Apigenin 7-O-glucuronide

S

56

C₅₀H₈₄O₂₃

[M−H]⁻

919.4982, 889.4860, 757.4376, 595.3851, 433.3344

Officinalisnin-II

As

57

C₄₅H₇₆O₁₉

[M−H]⁻

757.4378, 595.3847, 433.3319

3-O-β-d-glucopyranosyl (1 → 2)-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25S)-5β-furostane-3β,22α,26-triol

As

58

C₅₀H₈₄O₂₃

[M−H]⁻

919.4877, 889.4722, 757.4381, 594.6215, 418.5930

25-Epi-officinalisnin II

As

59

C₄₅H₇₆O₂₁

[M−H]⁻

633.9669, 475.0884

(2α,3β,5α,22S)-26-(β-d-Glucopyranosyloxy)-2,5,22-trihydroxyfurostan-3-yl 4-O-β-d-glucopyranosyl-β-d-glucopyranoside

As

60

C₄₅H₇₄O₁₇

[M−CO2−H]⁻

781.4773, 637.4346, 475.3789

Ginsenoside mRg1

P

61

C₄₈H₇₄O₂₀

[M−H]⁻

922.5041, 825.9856, 760.4498, 471.1639, 351.0573

(3β,22β)-22-(β-d-Glucopyranosyloxy)-11-oxoolean-12-en-3-yl 2-O-β-d-glucopyranuronosyl-β-d-glucopyranosiduronic acid

G

62

C₅₆H₉₂O₂₇

[M−H+HCOOH]⁻

1241.5817, 1195.5740, 1079.5382, 1033.5212, 917.4714, 755.4238, 455.1436

Ophiopojaponin G

O

63

C₂₁H₁₈O₁₁

[M−H]⁻

270.0491, 269.0456, 251.0346, 241.0503, 225.0552, 223.0392

Isomer of baicalin

S

64

C₄₅H₇₆O₁₉

[M−H]⁻

757.4382, 595.3838, 433.3329

3-O-β-d-Glucopyranosyl (1 → 2)-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25R)-5β-furostane-3β,22α,26-triol

As

65

C₅₁H₈₆O₂₄

[M−H]⁻

919.4806, 757.4385, 595.3859

26-(Hexopyranosyloxy)-22-hydroxyfurostan-3-yl hexopyranosyl-(1 → 2)hexopyranosyl-(1 → 4)hexopyranoside

A

66

C₂₁H₁₈O₁₀

[M−H]⁻

253.0505, 175.0236, 113.0229

Chrysin-7-O-β-d-glucuronid

S

67

C₂₂H₂₀O₁₁

[M−H]⁻

283.0614, 269.0411, 268.0377, 241.0481, 175.0235

Oroxylin A-7-O-β-d-glucuronide

S

68

C₅₁H₈₄O₂₃

[M−H]⁻

901.4807, 755.4263, 468.3537, 423.1946

Timosaponin BIV

A

69

C₂₂H₂₀O₁₂

[M−H]⁻

299.0563, 284.0327

Isomer of hydroxylwogonin glucuronide

S

70

C₅₆H₉₂O₂₈

[M−H]⁻

1079.5255, 917.4763, 865.0001, 755.4222

Timosaponin C1

A

71

C₄₅H₇₆O₁₉

[M−H]⁻

841.4293, 757.4416, 595.3847, 459.0930

Timosaponin BII

A

72

C₂₂H₂₀O₁₁

[M−H]⁻

283.0614, 269.0431, 268.0378, 240.0425, 175.0237

Wogonoside

S

73

C₅₇H₉₄O₂₇

[M−H]⁻

1047.5446, 901.4795, 883.4755, 755.4213, 737.4127, 431.3182

(2α,3β,5α,25R)-2-Hydroxyspirostan-3-yl β-d-glucopyranosyl-(1 → 2)-[4-O-[(2S,3R,4S)-3-hydroxy-4-(hydroxymethyl)-4-methyltetrahydro-2-furanyl]-β-d-glucopyranosyl-(1 → 3)]-β-d-glucopyranosyl-(1 → 4)-β-d-galactopyranoside

A

74

C₅₁H₈₄O₂₂

[M−H]⁻

901.4722, 885.4497, 755.4229

Protoneodioscin

As

75

C₅₁H₈₄O₂₂

[M−H]⁻

901.4749, 883.4813, 755.4178, 413.2992

Protodioscin

As

76

C₅₀H₈₄O₂₅

[M−H]⁻

1047.5375, 901.4825, 802.9248, 755.4275, 487.1885

(2α,3β,5α,22S,25R)-26-(β-d-Glucopyranosyloxy)-2,5,22-trihydroxyfurostan-3-yl β-d-xylopyranosyl-(1- > 3)-β-d-glucopyranosyl-(1- > 4)-β-d-galactopyranoside

As

77

C₅₀H₈₄O₂₂

[M−H]⁻

903.5004, 889.4836, 757.4378, 595.3881, 433.3307

3-O-α-l-Rhamnopyranosyl(1 → 4)-[β-d-xylopyranosyl (1 → 2)]-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25S)-5β-furostane-3β,22α,26-trio

As

78

C₅₁H₈₆O₂₃

[M−H]⁻

903.4990, 757.4362, 595.3870, 445.8120

(5α,22R)-26-(β-d-Glucopyranosyloxy)-22-hydroxyfurostan-3-yl 6-deoxy-α-l-mannopyranosyl-(1 → 4)-[α-l-mannopyranosyl-(1 → 2)]-β-d-glucopyranoside

A

79

C₄₈H₇₂O₂₂

[M−H]⁻

837.3885, 351.0569

24-hydroxy-licoricesaponin A3

G

80

C₅₁H₈₆O₂₃

[M−H]⁻

903.4954, 757.4395, 739.4266, 595.3826, 433.3332

3-O-α-l-rhamnopyranosyl (1 → 4)-[β-d-glucopyranosyl(1 → 2)]-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl(25R)-5β-furostane-3β,22α,26-triol

As

81

C₄₅H₇₆O₁₈

[M−H]⁻

757.4323, 595.3828, 433.3293

Aspacochioside A

As

82

C₄₅H₇₆O₁₈

[M−H]⁻

757.4388, 595.3868, 433.3327

Isomer of aspacochioside A

As

83

C₄₄H₆₄O₁₉

[M−H]⁻

456.4406, 429.6882, 351.0563

Hydroxy acetoxyglycyrrhizin

G

84

C₄₂H₆₂O₁₈

[M−H]⁻

351.0568

22-Hydroxy-licoricesaponin G2

G

85

C₄₈H₇₂O₂₁

[M−H]⁻

821.3983, 351.0575

Licorice-saponin A3

G

86

C₄₂H₆₀O₁₇

[M−H]⁻

801.4187, 443.5862, 381.5747, 351.0575

formylglycyrrhizin acid

G

87

C₅₀H₈₂O₂₂

[M−H]⁻

901.4716, 739.4283, 577.3704, 427.2860

3-O-β-d-xylopyranosyl (1 → 4)-[β-d-glucopyranosyl (1 → 2)]-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25S)-5β-furostane-20 (22)-ene-3β,26-diol

As

88

C₄₄H₆₄O₁₈

[M−H]⁻

351.0570, 193.0346, 175.0236, 113.0229

22β-Acetoxyglycyrrhizin

G

89

C₄₅H₇₄O₁₈

[M−H]⁻

739.4278, 577.3749, 356.5983

Xilingsaponin B

A

90

C₄₂H₆₂O₁₇

[M−H]⁻

351.0570

Licorice-saponin P2

G

91

C₅₁H₈₄O₂₃

[M−H]⁻

901.4749, 739.4255, 577.3785, 445.3186

3-O-β-d-xylopyranosyl(1 → 4)-[β-d-glucopyranosyl(1 → 2)-β-d-glucopyranosyl(1 → 2)]-β-d-glucopyranosyl-26-O-β-d-glucopyranosyl-(25S)-furostane-5-ene-22-methoxy-3β,26-diol

As

92

C₅₇H₉₄O₂₆

[M-CO2-H]⁻

1149.6069, 1107.5963, 945.5444, 783.4910, 621.4361, 459.3843

Ginsenoside mRb1

P

93

C₄₇H₈₀O₁₇

[M-H+HCOOH]⁻

919.4950, 758.4404, 497.1143, 435.1156

Gypenoside IX

P

94

C₄₈H₇₆O₁₉

[M−H]⁻

793.4381, 731.4389, 613.3751, 569.3850, 523.3790, 455.3533

Ginsenoside Ro

P

95

C₅₆H₉₂O₂₅

[M−CO2−H]⁻

1077.5857, 945.5567, 915.5332, 783.4905, 621.4422, 459.3855

Ginsenoside mRb2

P

96

C₄₅H₇₄O₁₇

[M−H]⁻

739.4278, 577.3763, 484.2304

3-O-[{α-L-rhamnopyranosyl-(1 → 4)}{β-d-glucopyranosyl}]-26-O-[β-d-glucopyranosyl](25S)-5β-furost-20(22)-en-3β,26-diol

As

97

C₄₂H₆₀O₁₆

[M−H]⁻

351.0568, 193.0346, 175.0237, 113.0229

Licorice-saponin E2

G

98

C₄₄H₆₀O₁₇

[M−H]⁻

837.3852, 797.3743, 351.0557

Methyllicorice-saponin Q2

G

99

C₄₂H₆₂O₁₇

[M−H]⁻

351.0570

Macedonoside A

G

100

C₄₈H₇₂O₂₀

[M−H]⁻

645.3641, 541.9834, 497.1151, 435.1159

(3β,22β)-23-Hydroxy-29-oxo-22,29-epoxyolean-12-en-3-yl 6-deoxy-α-L-mannopyranosyl-(1 → 2)-β-d-glucopyranuronosyl-(1 → 2)-β-d-glucopyranosiduronic acid

G

101

C₄₄H₆₄O₁₇

[M−H]⁻

351.0566, 193.0343, 175.0241, 113.0229

22β-Acetoxyglycyrrhaldehyde

G

102

C₄₂H₆₂O₁₇

[M−H]⁻

351.0568

Licorice-saponin Q2

G

103

C₃₉H₆₆O₁₄

[M−H]⁻

595.3846, 465.2493, 357.2007, 271.7701, 161.0441

Anemarrhenasaponin I or II

A

104

C₄₂H₆₂O₁₆

[M−H]⁻

352.0605, 351.0569, 193.0346, 175.0237, 113.0229

Glycyrrhizin acid

G

105

C₄₂H₆₄O₁₆

[M−H]⁻

721.3478, 351.0557, 193.0344, 175.0237, 113.0226

Licorice-saponin J2

G

106

C₄₂H₆₂O₁₇

[M−H]⁻

351.0573

Licorice-saponin G2

G

107

C₃₉H₆₂O₁₄

[M−H+HCOOH]⁻

799.4072, 753.4070, 205.0709, 163.0600, 119.0335

Ophiopojaponin Ra

O

108

C₄₂H₆₄O₁₅

[M−H]⁻

351.0572, 193.0346, 175.0236, 113.0230

Licorice-saponin B2

G

109

C₄₂H₆₂O₁₆

[M−H]⁻

352.0600, 351.0571, 193.0346, 175.0237, 113.0229

Licorice-saponin H2

G

110

C₃₉H₆₄O₁₄

[M−H]⁻

593.3716, 201.9089

Timosaponin AII

A

111

C₄₂H₆₂O₁₆

[M−H]⁻

352.0616, 351.0570, 193.0346, 175.0238, 113.0229

Licorice-saponin K2

G

112

C₄₂H₆₂O₁₆

[M−H]⁻

352.0634, 351.0573, 193.0345, 175.0237, 113.0229

Apioglycyrrhizin

G

113

C₄₂H₆₂O₁₅

[M−H]⁻

351.0572, 193.0349, 175.0240, 113.0229

Licorice-saponin C2

G