Table 2 Parameters of dianthrones of 6 analytes and 1 internal standard in MRM analysis
No. | Compounds | Retention times (RT, min) | Precursor ion (m/z) | Product ion (m/z) | Fragmentor voltage (FV) | Collision energy (CE) | Ion mode |
|---|---|---|---|---|---|---|---|
1 | Polygonumnolide C4 | 6.99 | 670.7 [M−H]− | 415.8 | 100 | 25 | (−) ESI |
2 | Polygonumnolide C3 | 7.63 | 671.0 [M−H]− | 415.8 | 100 | 25 | (−) ESI |
3 | Polygonumnolide C1 | 11.80 | 670.9 [M−H]− | 415.9 | 100 | 25 | (−) ESI |
4 | Polygonumnolide C2 | 13.25 | 670.9 [M−H]− | 416.0 | 100 | 25 | (−) ESI |
5 | trans-Emodin dianthrones | 16.51 | 508.8 [M−H]− | 253.8 | 150 | 25 | (−) ESI |
6 | cis-Emodin dianthrones | 17.06 | 508.7 [M−H]− | 253.9 | 150 | 25 | (−) ESI |
1S | Bianthronyl | 17.37 | 384.9 [M−H]− | 191.8 | 100 | 30 | (−) ESI |