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Table 4 Stability, repeatability, precision and recovery of 6 compounds

From: UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity

No

Stability

RSD (%) (n = 6)

Repeatability

RSD (%) (n = 6)

Precision

Recovery (n = 6)

Intra-day

RSD (%) (n = 6)

Inter-day

RSD (%) (n = 3)

Sample (g)

Original (µg)

Spiked (µg)

Found (µg)

Recovery (%)

Average recovery (%)

RSD (%)

1

3.41

3.12

2.73

2.85

0.502

1.009

1.092

2.383

125.87

  

0.502

1.009

1.092

2.654

150.67

  

0.503

1.011

1.092

2.418

128.81

134.46

7.91

0.512

1.029

1.092

2.583

142.33

  

0.501

1.007

1.092

2.494

136.14

  

0.518

1.041

1.092

2.383

122.92

  

2

3.55

3.24

1.68

2.79

0.502

0.512

0.535

1.184

125.61

  

0.502

0.512

0.535

1.318

150.65

  

0.503

0.513

0.535

1.203

128.97

  

0.512

0.522

0.535

1.328

150.65

134.05

9.70

0.501

0.511

0.535

1.183

125.61

  

0.518

0.528

0.535

1.185

122.80

  

3

3.64

1.92

2.58

2.12

0.502

0.512

0.594

1.345

140.29

  

0.502

0.512

0.594

1.430

154.48

  

0.503

0.513

0.594

1.390

147.60

  

0.512

0.522

0.594

1.454

156.79

150.04

3.88

0.501

0.511

0.594

1.398

149.35

  

0.518

0.528

0.594

1.430

151.73

  

4

3.47

1.23

2.22

4.05

0.502

0.638

0.672

1.505

129.15

  

0.502

0.638

0.672

1.674

154.28

  

0.503

0.639

0.672

1.492

126.94

143.88

8.68

0.512

0.650

0.672

1.683

153.70

  

0.501

0.636

0.672

1.630

147.93

  

0.518

0.658

0.672

1.674

151.26

  

5

3.95

1.97

2.11

4.63

0.502

2.113

2.131

4.159

96.01

  

0.502

2.113

2.131

4.457

109.99

  

0.503

2.118

2.131

4.230

99.11

  

0.512

2.156

2.131

4.430

106.71

105.53

9.65

0.501

2.109

2.131

4.201

98.17

  

0.518

2.181

2.131

4.806

123.18

  

6

3.73

3.30

1.67

2.90

0.502

1.531

1.512

3.051

100.52

  

0.502

1.531

1.512

3.092

103.20

  

0.503

1.534

1.512

3.106

103.98

  

0.512

1.562

1.512

3.289

114.24

104.38

6.19

0.501

1.528

1.512

2.974

95.66

  

0.518

1.580

1.512

3.224

108.71

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