UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity

Yang, Jian-Bo; Song, Yun-Fei; Liu, Yue; Gao, Hui-Yu; Wang, Qi; Wang, Ying; Cheng, Xian-Long; Zuo, Tian-Tian; Hu, Xiao-Wen; Wei, Feng; Jin, Hong-Tao; Wang, Shu-Ting; Ma, Shuang-Cheng

doi:10.1186/s13020-021-00463-w

Table 8 Contents of 6 dianthrones in 86 batches of Polygoni Multiflori Radix Praeparata (PMRP)

From: UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity

Sample no.	Contents of analytes (µg/g, n = 2^a)
	1	2	3	4	5	6	Total
	Mean ± (SD%)^c	Mean ± (SD%)^c	Mean ± (SD%)^c	Mean ± (SD%)^c	Mean ± (SD%)^c	Mean ± (SD%)^c	Total
PMRP-01	0.60 ± 1.32	3.36 ± 1.60	0.76 ± 1.04	2.57 ± 5.40	2.41 ± 6.96	2.16 ± 5.87	11.86
PMRP-02	0.051 ± 0.56	0.070 ± 0.98	0.064 ± 0.61	0.069 ± 0.73	0.41 ± 1.10	0.36 ± 0.74	1.02
PMRP-03	0.25 ± 0.42	0.45 ± 1.68	0.26 ± 1.15	0.60 ± 0.79	4.50 ± 12.09	3.38 ± 5.03	9.44
PMRP-04	0.051 ± 0.04	0.078 ± 0.15	0.071 ± 0.26	0.11 ± 0.59	0.35 ± 0.66	0.36 ± 0.78	1.02
PMRP-05	0.17 ± 0.23	0.23 ± 0.33	0.18 ± 0.11	0.35 ± 0.29	7.05 ± 17.31	6.70 ± 6.99	14.68
PMRP-06	0.081 ± 0.25	0.071 ± 0.09	0.084 ± 0.26	0.082 ± 0.17	5.47 ± 0.75	4.60 ± 17.77	10.39
PMRP-07	0.12 ± 0.14	0.19 ± 0.11	0.13 ± 0.11	0.28 ± 0.04	0.36 ± 0.83	0.37 ± 1.62	1.45
PMRP-08	0.16 ± 0.49	0.36 ± 0.70	0.19 ± 0.11	0.47 ± 0.06	7.21 ± 8.07	5.10 ± 27.48	13.49
PMRP-09	0.16 ± 0.09	0.47 ± 1.30	0.18 ± 0.57	0.42 ± 0.48	6.30 ± 9.12	6.09 ± 18.05	13.62
PMRP-10	0.079 ± 0.09	0.13 ± 0.24	0.094 ± 0.02	0.17 ± 0.99	0.19 ± 0.17	0.24 ± 0.61	0.90
PMRP-11	7.46 ± 2.35	5.73 ± 3.87	4.23 ± 0.28	7.36 ± 4.25	9.47 ± 4.41	8.05 ± 38.33	42.30
PMRP-12	0.10 ± 0.06	0.34 ± 0.26	0.11 ± 0.07	0.27 ± 0.19	1.25 ± 0.88	1.23 ± 2.59	3.30
PMRP-13	0.030 ± 0.00	^bND	0.05 ± 0.00	0.031 ± 0.00	0.098 ± 0.12	0.14 ± 0.58	0.35
PMRP-14	0.090 ± 0.22	0.13 ± 0.65	0.11 ± 0.40	0.16 ± 0.30	2.54 ± 1.95	2.27 ± 2.78	5.30
PMRP-15	3.51 ± 4.24	3.08 ± 2.97	2.47 ± 3.51	4.37 ± 9.27	2.68 ± 5.17	2.12 ± 4.77	18.23
PMRP-16	0.062 ± 0.05	0.095 ± 0.15	0.072 ± 0.34	0.085 ± 0.17	4.08 ± 5.99	3.99 ± 3.69	8.38
PMRP-17	0.070 ± 0.35	0.088 ± 0.23	0.081 ± 0.02	0.10 ± 0.34	0.62 ± 2.46	0.62 ± 1.38	1.58
PMRP-18	0.047 ± 0.26	0.082 ± 0.37	0.067 ± 0.26	0.098 ± 0.22	0.24 ± 0.20	0.27 ± 0.31	0.80
PMRP-19	4.42 ± 2.17	4.67 ± 4.59	2.36 ± 5.45	6.42 ± 4.79	8.68 ± 7.05	8.38 ± 28.49	34.93
PMRP-20	0.51 ± 0.27	0.44 ± 0.35	0.67 ± 0.15	0.64 ± 0.10	28.54 ± 7.99	25.95 ± 5.23	56.75
PMRP-21	0.047 ± 0.26	0.051 ± 0.19	0.074 ± 0.55	0.063 ± 0.65	0.52 ± 0.61	0.45 ± 0.39	1.21
PMRP-22	0.22 ± 0.12	0.29 ± 0.00	0.20 ± 0.00	0.40 ± 0.32	9.31 ± 0.00	8.62 ± 0.00	19.04
PMRP-23	0.030 ± 0.08	^bND	0.050 ± 0.00	0.030 ± 0.45	0.19 ± 2.99	0.21 ± 2.62	0.51
PMRP-24	0.77 ± 0.99	0.64 ± 0.49	0.46 ± 1.93	0.99 ± 1.74	8.87 ± 10.19	8.22 ± 2.72	19.95
PMRP-25	0.14 ± 0.17	0.28 ± 0.44	0.18 ± 0.62	0.34 ± 0.92	2.81 ± 12.24	2.02 ± 4.31	5.77
PMRP-26	0.10 ± 0.06	0.068 ± 0.04	0.095 ± 0.37	0.098 ± 0.11	0.71 ± 0.52	0.63 ± 1.47	1.70
PMRP-27	0.26 ± 0.59	0.42 ± 0.96	0.27 ± 0.02	0.67 ± 1.75	6.52 ± 5.11	5.13 ± 19.97	13.27
PMRP-28	1.18 ± 0.03	1.75 ± 0.53	1.35 ± 0.05	2.35 ± 0.45	13.77 ± 7.12	10.30 ± 6.31	30.7
PMRP-29	0.063 ± 0.11	0.14 ± 0.39	0.091 ± 0.07	0.16 ± 0.52	0.95 ± 5.51	0.71 ± 2.44	2.11
PMRP-30	0.054 ± 0.29	0.16 ± 0.60	0.098 ± 1.79	0.12 ± 0.08	1.53 ± 3.12	1.19 ± 3.69	3.15
PMRP-31	9.37 ± 8.79	6.32 ± 11.82	5.23 ± 7.88	11.36 ± 14.95	8.23 ± 1.64	7.79 ± 6.42	48.30
PMRP-32	3.48 ± 1.90	2.02 ± 0.60	1.70 ± 1.63	3.40 ± 1.31	6.73 ± 3.92	5.92 ± 22.20	23.25
PMRP-33	0.065 ± 0.41	0.094 ± 0.21	0.084 ± 0.28	0.12 ± 0.19	0.59 ± 5.10	0.51 ± 3.46	1.46
PMRP-34	0.081 ± 0.57	0.10 ± 0.58	0.085 ± 0.27	0.12 ± 0.59	0.89 ± 3.39	0.70 ± 3.10	1.98
PMRP-35	0.16 ± 0.81	0.23 ± 0.18	0.17 ± 0.30	0.41 ± 1.48	0.32 ± 1.99	0.35 ± 1.18	1.64
PMRP-36	0.15 ± 0.25	0.27 ± 0.02	0.17 ± 0.07	0.34 ± 0.03	0.31 ± 0.03	3.52 ± 9.47	4.76
PMRP-37	0.11 ± 0.02	0.34 ± 0.14	0.12 ± 0.09	0.35 ± 0.49	0.33 ± 1.51	0.31 ± 1.16	1.56
PMRP-38	0.056 ± 0.01	0.075 ± 0.29	0.071 ± 0.21	0.089 ± 0.01	0.28 ± 0.15	0.30 ± 1.38	0.87
PMRP-39	0.21 ± 0.64	0.53 ± 0.51	0.23 ± 0.15	0.59 ± 1.60	3.95 ± 12.81	2.67 ± 2.57	8.18
PMRP-40	0.057 ± 0.08	0.18 ± 0.83	0.072 ± 0.15	0.12 ± 0.36	3.24 ± 9.23	2.66 ± 3.43	6.33
PMRP-41	0.025 ± 0.29	0.051 ± 0.19	0.039 ± 0.13	0.063 ± 0.31	0.38 ± 0.63	0.27 ± 0.86	0.83
PMRP-42	^bND	0.089 ± 0.00	^bND	0.14 ± 0.02	0.36 ± 2.36	0.24 ± 0.90	0.83
PMRP-43	0.13 ± 0.028	0.39 ± 0.32	0.16 ± 1.60	0.62 ± 0.53	1.42 ± 10.91	1.13 ± 14.98	3.85
PMRP-44	0.025 ± 0.08	0.17 ± 0.061	0.054 ± 0.13	0.23 ± 0.11	0.32 ± 0.70	0.21 ± 0.15	1.01
PMRP-45	^bND	0.089 ± 0.043	^bND	0.14 ± 0.00	0.33 ± 0.26	0.24 ± 0.71	0.80
PMRP-46	0.028 ± 0.17	0.48 ± 0.028	0.14 ± 0.37	0.56 ± 0.90	3.25 ± 16.03	3.29 ± 7.35	7.86
PMRP-47	0.08 ± 0.16	0.411 ± 0.08	0.073 ± 0.44	0.32 ± 0.14	0.40 ± 1.05	0.28 ± 0.35	1.53
PMRP-48	0.42 ± 0.04	1.63 ± 1.42	0.69 ± 0.28	2.68 ± 0.31	9.86 ± 0.14	7.38 ± 1.26	22.66
PMRP-49	0.20 ± 0.03	1.21 ± 0.04	0.36 ± 0.00	1.59 ± 0.02	26.99 ± 10.44	27.79 ± 0.27	58.14
PMRP-50	7.40 ± 2.13	5.61 ± 0.37	3.94 ± 0.39	10.31 ± 1.79	11.01 ± 0.97	10.85 ± 0.43	49.12
PMRP-51	0.027 ± 0.38	0.13 ± 0.01	0.040 ± 0.07	0.17 ± 0.18	1.90 ± 1.42	1.53 ± 0.53	3.80
PMRP-52	13.03 ± 18.67	8.94 ± 20.21	7.23 ± 10.11	12.75 ± 16.48	10.82 ± 61.54	12.50 ± 6.99	65.27
PMRP-53	0.46 ± 0.41	1.06 ± 3.01	0.54 ± 0.34	1.35 ± 3.72	1.26 ± 3.35	1.41 ± 2.37	6.08
PMRP-54	0.16 ± 0.08	0.54 ± 1.02	0.20 ± 0.36	0.64 ± 0.52	5.29 ± 7.85	5.50 ± 14.07	12.33
PMRP-55	0.072 ± 0.08	0.36 ± 0.60	0.10 ± 0.03	0.41 ± 0.64	1.81 ± 4.11	1.80 ± 1.06	4.55
PMRP-56	8.25 ± 4.61	4.64 ± 5.20	4.95 ± 5.92	8.79 ± 10.87	3.80 ± 7.98	4.60 ± 2.97	35.03
PMRP-57	0.22 ± 0.78	0.58 ± 1.31	0.25 ± 0.63	0.79 ± 1.61	5.18 ± 12.17	4.65 ± 3.99	11.67
PMRP-58	0.40 ± 0.89	0.92 ± 4.89	0.55 ± 0.53	1.15 ± 0.59	3.24 ± 0.48	3.41 ± 6.09	9.67
PMRP-59	0.71 ± 0.64	0.87 ± 5.14	0.54 ± 0.26	0.92 ± 1.15	16.79 ± 24.06	18.91 ± 70.89	38.74
PMRP-60	5.61 ± 0.64	8.19 ± 2.47	3.68 ± 0.55	10.28 ± 0.99	18.71 ± 9.53	18.75 ± 7.29	65.22
PMRP-61	0.032 ± 0.18	0.17 ± 0.24	0.054 ± 0.23	0.21 ± 0.66	0.29 ± 2.00	0.18 ± 1.71	0.94
PMRP-62	0.095 ± 0.10	0.16 ± 0.75	0.069 ± 0.43	0.25 ± 0.27	0.34 ± 3.00	0.24 ± 1.83	1.15
PMRP-63	0.13 ± 0.07	0.57 ± 0.69	0.13 ± 0.09	0.49 ± 0.13	0.56 ± 0.41	0.46 ± 1.84	2.34
PMRP-64	^bND	0.12 ± 0.43	0.032 ± 0.10	0.17 ± 0.48	0.26 ± 0.62	0.15 ± 0.60	0.73
PMRP-65	0.028 ± 0.17	0.11 ± 0.12	0.044 ± 0.04	0.17 ± 0.27	0.49 ± 0.31	0.40 ± 1.86	1.24
PMRP-66	0.08 ± 0.36	0.15 ± 0.10	0.040 ± 0.24	0.20 ± 1.23	0.28 ± 0.09	0.19 ± 0.92	0.89
PMRP-67	0.52 ± 1.64	0.36 ± 0.38	0.31 ± 0.04	0.73 ± 1.15	0.38 ± 0.14	0.31 ± 0.58	2.61
PMRP-68	^bND	0.090 ± 0.01	0.022 ± 0.078	0.14 ± 0.080	0.31 ± 0.95	0.18 ± 0.34	0.74
PMRP-69	0.028 ± 0.16	0.20 ± 0.56	0.080 ± 0.03	0.27 ± 0.42	1.36 ± 2.16	1.45 ± 4.49	3.41
PMRP-70	^bND	0.089 ± 0.01	0.022 ± 0.019	0.14 ± 0.00	0.48 ± 0.49	0.38 ± 0.40	1.11
PMRP-71	1.19 ± 4.47	0.85 ± 1.93	0.89 ± 3.43	0.81 ± 4.23	2.32 ± 18.15	2.21 ± 17.49	8.27
PMRP-72	0.40 ± 1.45	1.21 ± 4.70	0.50 ± 0.19	1.52 ± 2.72	6.52 ± 22.57	5.29 ± 18.23	15.44
PMRP-73	0.45 ± 0.29	0.78 ± 1.43	0.47 ± 0.79	1.21 ± 0.37	6.23 ± 10.35	5.98 ± 16.38	15.12
PMRP-74	0.054 ± 0.03	0.19 ± 0.53	0.059 ± 0.18	0.23 ± 0.39	3.41 ± 2.97	3.25 ± 2.33	7.19
PMRP-75	0.029 ± 0.14	1.25 ± 0.29	0.36 ± 0.24	1.71 ± 0.16	10.67 ± 0.60	10.10 ± 1.88	24.12
PMRP-76	0.55 ± 0.50	0.43 ± 0.20	0.45 ± 0.81	0.51 ± 0.51	1.20 ± 0.79	1.14 ± 0.16	4.28
PMRP-77	1.15 ± 1.12	0.97 ± 0.54	0.98 ± 2.83	1.00 ± 0.48	6.13 ± 11.15	5.36 ± 37.35	15.59
PMRP-78	1.18 ± 0.21	0.95 ± 3.74	1.03 ± 0.82	0.97 ± 1.22	6.23 ± 9.83	5.37 ± 15.03	15.73
PMRP-79	1.34 ± 0.75	1.12 ± 2.17	1.15 ± 1.72	1.08 ± 3.02	6.22 ± 5.22	5.48 ± 4.66	16.39
PMRP-80	1.30 ± 0.68	0.91 ± 8.23	0.90 ± 0.99	1.48 ± 1.62	1.41 ± 12.83	1.44 ± 3.38	7.44
PMRP-81	0.20 ± 0.39	0.47 ± 1.71	0.22 ± 0.93	0.73 ± 2.19	1.73 ± 1.46	1.44 ± 3.51	4.79
PMRP-82	^bND	0.090 ± 0.01	0.022 ± 0.081	0.14 ± 0.12	0.38 ± 0.60	0.27 ± 0.45	0.90
PMRP-83	0.19 ± 0.20	3.13 ± 1.39	1.84 ± 0.42	4.48 ± 1.85	19.83 ± 2.04	21.69 ± 1.97	51.16
PMRP-84	0.849 ± 1.01	1.03 ± 0.79	0.76 ± 1.06	1.73 ± 3.45	5.40 ± 12.61	5.86 ± 22.91	15.63
PMRP-85	0.269 ± 1.38	0.51 ± 0.96	0.26 ± 0.15	0.71 ± 1.08	1.38 ± 0.96	1.29 ± 0.34	4.42
PMRP-86	0.65 ± 1.37	6.80 ± 1.29	3.53 ± 0.35	9.55 ± 3.24	15.04 ± 3.08	15.83 ± 0.01	51.40
Average	0.96	1.08	0.71	1.53	4.47	4.25	12.99

^aThe data are presented as the average of two replicates
^bND, under limits of quantitation
^cSD% is presented in the table

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