Fig. 3

SZJN increased clathrin and RAB5B expression, whereas reduced cytomembrane NMDAR1 expression in the Hippocampus of VD Rats. A Immunohistochemical analysis of clathrin, RAB5B, and NMDAR1 expression in hippocampal CA1 regions (×400). Quantitative analysis showed the expression of B clathrin, C RAB5B, and D NMDAR1 in hippocampal CA1 regions. E Western blot was performed to detect the levels of clathrin, RAB5B, cytoplasmic NMDAR1, and cytomembrane NMDAR1 in the hippocampus of VD rats. Quantitative analysis of F clathrin, G RAB5B, H cytoplasmic NMDAR1, and I cytomembrane NMDAR1 expression. The densities of the bands were normalized with respect to the values of β-actin. qRT-PCR analyzed J clathrin mRNA and K NMDAR1 mRNA expression levels. Values were expressed as means ± SD (n = 6). ^*P < 0.05, ^**P < 0.01 versus sham-surgery group. ^#P < 0.05, ^##P < 0.01 versus VD group