Fig. 4

Effects of SZJN formula on glutamate-induced cytotoxicity to PC12 cells. A Effects of SZJN formula (0.025, 0.05, 0.1, 0.2, and 0.4 mg/mL) on basal growth of PC12 cells. B Effects of glutamate (22, 22.5 and 23 mM) on PC12 cell viability. C Effects of glutamate (22, 22.5 and 23 mM) on the area of total green fluorescence. D Effects of SZJN formula on cell viability, E number of dead cells, F green fluorescent intensity, and G green fluorescent area in glutamate-exposed PC12 cells. Following treatment of the cells with various concentrations of SZJN formula (0.05, 0.1, and 0.2 mg/mL) for 24 h and exposure to 22.5 mM of glutamate for 24 h. H Effects of SZJN formula on cell viability using CCK8 assay. I Effects of SZJN formula on LDH release in PC12 cells. J Effects of SZJN formula in glutamate-induced cell morphological changes. SZJN-L: 0.05 mg/mL, SZJN-M: 0.1 mg/mL, SZJN-H: 0.2 mg/mL. Values were expressed as means ± SD (n = 4). ^*P < 0.05, ^**P < 0.01 as compared with the control group. ^#P < 0.05, ^##P < 0.01 as compared with the glutamate group