Fig. 6

Effects of SZJN formula on the protein and mRNA expressions of clathrin, RAB5B, and NMDAR1. A Western blot was performed to detect the protein levels of clathrin, RAB5B, and NMDAR1 in glutamate treated PC12 cells. Quantitative analysis of B clathrin, C RAB5B, and D NMDAR1 expressions. β-Actin was used as the internal control. qRT-PCR analyzed E clathrin mRNA and F NMDAR1 mRNA expression levels. SZJN-L: 0.05 mg/mL, SZJN-M: 0.1 mg/mL, SZJN-H: 0.2 mg/mL. Values were expressed as means ± SD (n = 3). ^*P < 0.05, ^**P < 0.01 as compared with the control group. ^#P < 0.05, ^##P < 0.01 as compared with the glutamate group