Fig. 7

The promotion of SZJN formula on the process of CME could be blocked by CME inhibitors. A Western blot was performed to detect the protein levels of clathrin, RAB5B, and NMDAR1 in glutamate-injured PC12 cells after treatment with CPZ or SZJN formula (0.1 mg/mL). β-Actin was used as the internal control. B qRT-PCR analyzed clathrin mRNA and NMDAR1 mRNA expression levels in glutamate-injured PC12 cells after treatment with CPZ or SZJN formula (0.1 mg/mL). C Western blot was performed to detect the protein levels of clathrin, RAB5B, and NMDAR1 in glutamate-injured PC12 cells after treatment with Pitstop 2 or SZJN formula (0.1 mg/mL). β-Actin was used as the internal control. D qRT-PCR analyzed clathrin mRNA and NMDAR1 mRNA expression levels in glutamate-injured PC12 cells after treatment with Pitstop 2 or SZJN formula (0.1 mg/mL). Values were expressed as means ± SD (n = 3). ^*P < 0.05, ^**P < 0.01 as compared with the control group. ^#P < 0.05, ^##P < 0.01 as compared with the glutamate group. ^&P < 0.05, ^&&P < 0.01 as compared with the SZJN-M group