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Table 2 Antioxidant activities of E. ulmoides

From: Traditional application and modern pharmacological research of Eucommia ulmoides Oliv.

Compounds

Subjects

Chemical composition

Effects

Notes

References

Ethanol extract of E. ulmoides leaf (EEEuL)

DPPH radical- cavenging; Fe2+-chelating ability;

Lipid peroxidation

Total phenolic content: mg CAE/g. Geniposidic acid: 2.14 ± 0.04 mg/g. Epicatechin: 1.93 ± 0.05 mg/g.

Chlorogenic acid: 8.45 ± 0.9 mg/g.

EC50 of DPPH radical-scavenging: 1.35 ± 0.03 mg/ml

Total phenolic content as chlorogenic acid equivalents (CAE)

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EC50 of Fe2+-chelating ability: not detectable

EC50 of Lipid peroxidation: 16.74 ± 0.09 mg/ml

  

Ethanol extract of E. ulmoides leaf (EEEuL)

DPPH radical- scavenging; ferric reducing antioxidant power (FRAP); lipid peroxidation inhibition capacity

Total Phenolics Content (TPC):

94.46 ± 17 mg of GAE/g

Total Flavonoids Content (TFC):

61.36 ± 0.59 mg of CE/g. Chlorogenic acid: 18.39 ± 0.09 mg/g

Caffeic acid: 2.55 ± 0.08 mg/g

Protocatechuic acid: 1.60 ± 0.03 mg/g

Rutin: 9.99 ± 0.07 mg/g

Quercetin: 3.51 ± 0.05 mg/g

Kaempferol: 1.10 ± 0.04 mg/g

DPPH scavenging activity: 81.40 ± 3.63%

Ferric reducing power EC1: 0.72 ± 0.02 mg/ml

Lipid peroxidation inhibition capacity: 43.58 ± 3.29%

Total phenolic content as gallic acid (GAE) equivalents

Total Flavonoids Content (TFC) as catechin equivalents (CE) equivalents

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Ethanol extract of E. ulmoides roasted bark (EEEuRB)

 

Total Phenolics Content (TPC):

40.07 ± 0.45 mg of GAE/g

Total Flavonoids Content (TFC):

16.84 ± 0.20 mg of CE/g. Chlorogenic acid: 7.38 ± 0.12 mg/g

Caffeic acid: 1.54 ± 0.05 mg/g

Protocatechuic acid: 1.02 ± 0.06 mg/g

Rutin: 2.46 ± 0.05 mg/g

Quercetin: not detectable Kaempferol: not detectable

DPPH scavenging activity: 16.72 ± 0.25%

Ferric reducing power EC1: 2.81 ± 0.09 mg/ml

Lipid peroxidation inhibition capacity: 26.71 ± 2.21%

  

Ethanol extract of E. ulmoides seed (EEEuS)

 

Total Phenolics Content (TPC):

19.11 ± 0.12 mg of GAE/g

Total Flavonoids Content (TFC):

7.97 ± 0.11 mg of CE/g. Chlorogenic acid: 0.67 ± 0.01 mg/g

Caffeic acid: not detectable. Protocatechuic acid: 0.26 ± 0.03 mg/g

Rutin: not detectable. Quercetin: not detectable

Kaempferol: not detectable

DPPH scavenging activity: 7.65 ± 0.20%

Ferric reducing power EC1: 8.43 ± 0.75 mg/ml

Lipid peroxidation inhibition capacity: 25.10 ± 1.37%

  

Aqueous extract of E. ulmoides leaf (AEEuL)

DPPH radical- scavenging

Geniposidic acid Caffeic acid Chlorogenic acid Ferulic acid

Quercetin 3-O-sambubioside Rutin

Isoquercitrin Ascorbic acid

IC50 of AEEuL: 18.9 ± 0.2 μg/ml IC50 of Geniposidic acid: > 50 μg/ml

IC50 of Caffeic acid: 0.8 ± 0.1 μg/ml

IC50 of Chlorogenic acid: 2.3 ± 0.1 μg/ml

IC50 of Ferulic acid: 1.6 ± 0.1 μg/ml

IC50 of Quercetin 3-O- sambubioside: 5.7 ± 0.2 μg/ml IC50 of Rutin: 19.8 ± 0.2 μg/ml IC50 of Isoquercitrin: 7.2 ± 0.1 μg/ml

IC50 of Ascorbic acid: 5.9 ± 0.1 μg/ml

 

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