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Table 2 Antioxidant activities of E. ulmoides

From: Traditional application and modern pharmacological research of Eucommia ulmoides Oliv.

Compounds Subjects Chemical composition Effects Notes References
Ethanol extract of E. ulmoides leaf (EEEuL) DPPH radical- cavenging; Fe2+-chelating ability;
Lipid peroxidation
Total phenolic content: mg CAE/g. Geniposidic acid: 2.14 ± 0.04 mg/g. Epicatechin: 1.93 ± 0.05 mg/g.
Chlorogenic acid: 8.45 ± 0.9 mg/g.
EC50 of DPPH radical-scavenging: 1.35 ± 0.03 mg/ml Total phenolic content as chlorogenic acid equivalents (CAE) [53]
EC50 of Fe2+-chelating ability: not detectable
EC50 of Lipid peroxidation: 16.74 ± 0.09 mg/ml   
Ethanol extract of E. ulmoides leaf (EEEuL) DPPH radical- scavenging; ferric reducing antioxidant power (FRAP); lipid peroxidation inhibition capacity Total Phenolics Content (TPC):
94.46 ± 17 mg of GAE/g
Total Flavonoids Content (TFC):
61.36 ± 0.59 mg of CE/g. Chlorogenic acid: 18.39 ± 0.09 mg/g
Caffeic acid: 2.55 ± 0.08 mg/g
Protocatechuic acid: 1.60 ± 0.03 mg/g
Rutin: 9.99 ± 0.07 mg/g
Quercetin: 3.51 ± 0.05 mg/g
Kaempferol: 1.10 ± 0.04 mg/g
DPPH scavenging activity: 81.40 ± 3.63%
Ferric reducing power EC1: 0.72 ± 0.02 mg/ml
Lipid peroxidation inhibition capacity: 43.58 ± 3.29%
Total phenolic content as gallic acid (GAE) equivalents
Total Flavonoids Content (TFC) as catechin equivalents (CE) equivalents
[54]
Ethanol extract of E. ulmoides roasted bark (EEEuRB)   Total Phenolics Content (TPC):
40.07 ± 0.45 mg of GAE/g
Total Flavonoids Content (TFC):
16.84 ± 0.20 mg of CE/g. Chlorogenic acid: 7.38 ± 0.12 mg/g
Caffeic acid: 1.54 ± 0.05 mg/g
Protocatechuic acid: 1.02 ± 0.06 mg/g
Rutin: 2.46 ± 0.05 mg/g
Quercetin: not detectable Kaempferol: not detectable
DPPH scavenging activity: 16.72 ± 0.25%
Ferric reducing power EC1: 2.81 ± 0.09 mg/ml
Lipid peroxidation inhibition capacity: 26.71 ± 2.21%
  
Ethanol extract of E. ulmoides seed (EEEuS)   Total Phenolics Content (TPC):
19.11 ± 0.12 mg of GAE/g
Total Flavonoids Content (TFC):
7.97 ± 0.11 mg of CE/g. Chlorogenic acid: 0.67 ± 0.01 mg/g
Caffeic acid: not detectable. Protocatechuic acid: 0.26 ± 0.03 mg/g
Rutin: not detectable. Quercetin: not detectable
Kaempferol: not detectable
DPPH scavenging activity: 7.65 ± 0.20%
Ferric reducing power EC1: 8.43 ± 0.75 mg/ml
Lipid peroxidation inhibition capacity: 25.10 ± 1.37%
  
Aqueous extract of E. ulmoides leaf (AEEuL) DPPH radical- scavenging Geniposidic acid Caffeic acid Chlorogenic acid Ferulic acid
Quercetin 3-O-sambubioside Rutin
Isoquercitrin Ascorbic acid
IC50 of AEEuL: 18.9 ± 0.2 μg/ml IC50 of Geniposidic acid: > 50 μg/ml
IC50 of Caffeic acid: 0.8 ± 0.1 μg/ml
IC50 of Chlorogenic acid: 2.3 ± 0.1 μg/ml
IC50 of Ferulic acid: 1.6 ± 0.1 μg/ml
IC50 of Quercetin 3-O- sambubioside: 5.7 ± 0.2 μg/ml IC50 of Rutin: 19.8 ± 0.2 μg/ml IC50 of Isoquercitrin: 7.2 ± 0.1 μg/ml
IC50 of Ascorbic acid: 5.9 ± 0.1 μg/ml
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