Table 3 The gradient elution conditions of the mobile phase
Time (min) | Phase A (%) | Phase B (%) | Phase C (%) |
|---|---|---|---|
0–15 | 5–17 | 0–83 | 95–0 |
15–24 | 17–20 | 83–80 | 0 |
24–48 | 20–24 | 80–76 | 0 |
48–52 | 24–28 | 76–72 | 0 |
52–60 | 28–31 | 72–69 | 0 |
60–65 | 31–35 | 69–65 | 0 |
65–80 | 35–70 | 65–30 | 0 |
80–81 | 70–5 | 30–0 | 0–95 |
81–90 | 5 | 0 | 95 |