Fig. 12

Expression of TLR4/Iba1, TLR4/GFAP, and NF-κB/GFAP, and activated forms of Iba1 and GFAP in the penumbral cortex. Arrows in A-1, A-2, B-1, B-2, C-1, C-2, and C-4 indicate TLR4-, Iba1-, TLR4-, GFAP-, NF-κB-, GFAP-, and DAPI-positive cells, respectively. Arrows in A-3, B-3, and C-3 indicate TLR4/Iba1, TLR4/GFAP, and NF-κB/GFAP double-labeled cells, respectively. Arrows in C-5 indicate NF-κB/GFAP-positive cells labeled with DAPI. Arrowheads in A-2 and C-2 indicate activated forms of Iba1 and GFAP, respectively. The bar graphs showed the percentages of activated forms of D Iba1 and E GFAP in the penumbral cortex among the experimental groups (n = 5) at 1 day after reperfusion. In addition, the bar graphs showed the numbers of F TLR4/Iba1- G TLR4/GFAP-, and H NF-κB/GFAP-positive cells in the penumbral cortex among the experimental groups (n = 5). *P < 0.05 vs. the Sham group; ^#P < 0.05 vs. the Control group. Scale bars in B-3 and C-5 represent 50 μm