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Table 2 Primary and secondary antibodies applied in this study

From: Alpinia oxyphylla Miq extract reduces cerebral infarction by downregulating JNK-mediated TLR4/T3JAM- and ASK1-related inflammatory signaling in the acute phase of transient focal cerebral ischemia in rats

Source Primary antibody
Secondary antibody
WB
Dilution
IHC
Dilution
IF
Dilution
Supplier/product number
Rabbit
Goat
p-ASK1
Anti-rabbit IgG
1:1000
1:5000
   CST/#3765
Jackson/AB_2313567
Rabbit
Goat
ASK1
Anti-rabbit IgG
1:1000
1:5000
   CST/#8662
Jackson/AB_2313567
Rabbit
Goat
MyD88
Anti-rabbit IgG
1:1000
1:5000
   CST/#4283
Jackson/AB_2313567
Rabbit
Goat
TRAF6
Anti-rabbit IgG
1:1000
1:5000
   abcam/ab40675
Jackson/AB_2313567
Rabbit
Goat
T3JAM
Anti-rabbit IgG
1:500
1:5000
   Merck/SAB4503206
Jackson/AB_2313567
Rabbit
Goat
TRAF3
Anti-rabbit IgG
1:500
1:5000
   abcam/36988
Jackson/AB_2313567
Rabbit
Goat
Iba1
Anti-rabbit IgG
1:1000
1:5000
   abcam/ab178846
Jackson/AB_2313567
Rabbit
Goat
p-JNK
Anti-rabbit IgG
1:1000
1:5000
   CST/#9251
Jackson/AB_2313567
Rabbit
Goat
JNK
Anti-rabbit IgG
1:1000
1:5000
   CST/#9252
Jackson/AB_2313567
Rabbit
Goat
p-p38 MAPK
Anti-rabbit IgG
1:1000
1:5000
   CST/#9211
Jackson/AB_2313567
Rabbit
Goat
P38 MAPK
Anti-rabbit IgG
1:1000
1:5000
   CST/#9212
Jackson/AB_2313567
Rabbit
Goat
iNOS
Anti-rabbit IgG
1:250
1:5000
   abcam/ab15323
Jackson/AB_2313567
Rabbit
Goat
COX-2
Anti-rabbit IgG
1:1000
1:5000
   CST/#4842
Jackson/AB_2313567
Rabbit
Goat
TNF-α
Anti-rabbit IgG
1:1000
1:5000
   Millipore/AB1837P
Jackson/AB_2313567
Mouse
Goat
Actin (loading control)
Anti-mouse IgG
1:5000
1:5000
   NOVUS/NB600-501
Jackson/AB_10015289
Rabbit Iba1
Polymer kit
  1:500   abcam/ab178846
Leica/RE7111&RE7112
Mouse TLR4
Polymer kit
  1:100   abcam/ab22048
Leica/RE7111&RE7112
Mouse GFAP
Polymer kit
  1:200   CST/#3670
Leica/RE7111&RE7112
Rabbit NF-κB (p65)
Polymer kit
  1:200   abcam/ab16502
Leica/RE7111&RE7112
Rabbit iNOS
Polymer kit
  1:100   abcam/ab15323
Leica/RE7111&RE7112
Mouse IL-6
Polymer kit
  1:500   abcam/ab9324
Leica/RE7111&RE7112
Mouse
Goat
NeuN
Anti-mouse IgG
   1:100
1:100
abcam/ab178846
Jackson Alexa 594/AB_2338871
Rabbit
Goat
Iba1
Anti-rabbit IgG
   1:250
1:100
abcam/ab178846
Jackson Alexa 488/AB_2338046
Mouse
Goat
TLR4
Anti-mouse IgG
   1:100
1:100
abcam/ab22048
Jackson Alexa 594/AB_2338871
Mouse
Goat
GFAP
Anti-mouse IgG
   1:100
1:100
CST/#3670
Jackson Alexa 594/AB_2338871
Rabbit
Goat
NF-κB (p65)
Anti-rabbit IgG
   1:100
1:100
abcam/ab16502
Jackson Alexa 488/AB_2338046
Rabbit
Goat
GFAP
Anti-rabbit IgG
   1:100
1:100
abcam/ab104139
Jackson Alexa 488/AB_2338046
  1. WB: Western blotting; IHC: immunohistochemistry; IF: immunofluorescence; CST: Cell Signaling Technology