Table 2 Primary and secondary antibodies applied in this study
| Source |
Primary antibody Secondary antibody |
WB Dilution |
IHC Dilution |
IF Dilution | Supplier/product number |
|---|---|---|---|---|---|
|
Rabbit Goat |
p-ASK1 Anti-rabbit IgG |
1:1000 1:5000 |
CST/#3765 Jackson/AB_2313567 | ||
|
Rabbit Goat |
ASK1 Anti-rabbit IgG |
1:1000 1:5000 |
CST/#8662 Jackson/AB_2313567 | ||
|
Rabbit Goat |
MyD88 Anti-rabbit IgG |
1:1000 1:5000 |
CST/#4283 Jackson/AB_2313567 | ||
|
Rabbit Goat |
TRAF6 Anti-rabbit IgG |
1:1000 1:5000 |
abcam/ab40675 Jackson/AB_2313567 | ||
|
Rabbit Goat |
T3JAM Anti-rabbit IgG |
1:500 1:5000 |
Merck/SAB4503206 Jackson/AB_2313567 | ||
|
Rabbit Goat |
TRAF3 Anti-rabbit IgG |
1:500 1:5000 |
abcam/36988 Jackson/AB_2313567 | ||
|
Rabbit Goat |
Iba1 Anti-rabbit IgG |
1:1000 1:5000 |
abcam/ab178846 Jackson/AB_2313567 | ||
|
Rabbit Goat |
p-JNK Anti-rabbit IgG |
1:1000 1:5000 |
CST/#9251 Jackson/AB_2313567 | ||
|
Rabbit Goat |
JNK Anti-rabbit IgG |
1:1000 1:5000 |
CST/#9252 Jackson/AB_2313567 | ||
|
Rabbit Goat |
p-p38 MAPK Anti-rabbit IgG |
1:1000 1:5000 |
CST/#9211 Jackson/AB_2313567 | ||
|
Rabbit Goat |
P38 MAPK Anti-rabbit IgG |
1:1000 1:5000 |
CST/#9212 Jackson/AB_2313567 | ||
|
Rabbit Goat |
iNOS Anti-rabbit IgG |
1:250 1:5000 |
abcam/ab15323 Jackson/AB_2313567 | ||
|
Rabbit Goat |
COX-2 Anti-rabbit IgG |
1:1000 1:5000 |
CST/#4842 Jackson/AB_2313567 | ||
|
Rabbit Goat |
TNF-α Anti-rabbit IgG |
1:1000 1:5000 |
Millipore/AB1837P Jackson/AB_2313567 | ||
|
Mouse Goat |
Actin (loading control) Anti-mouse IgG |
1:5000 1:5000 |
NOVUS/NB600-501 Jackson/AB_10015289 | ||
| Rabbit |
Iba1 Polymer kit | 1:500 |
abcam/ab178846 Leica/RE7111&RE7112 | ||
| Mouse |
TLR4 Polymer kit | 1:100 |
abcam/ab22048 Leica/RE7111&RE7112 | ||
| Mouse |
GFAP Polymer kit | 1:200 |
CST/#3670 Leica/RE7111&RE7112 | ||
| Rabbit |
NF-κB (p65) Polymer kit | 1:200 |
abcam/ab16502 Leica/RE7111&RE7112 | ||
| Rabbit |
iNOS Polymer kit | 1:100 |
abcam/ab15323 Leica/RE7111&RE7112 | ||
| Mouse |
IL-6 Polymer kit | 1:500 |
abcam/ab9324 Leica/RE7111&RE7112 | ||
|
Mouse Goat |
NeuN Anti-mouse IgG |
1:100 1:100 |
abcam/ab178846 Jackson Alexa 594/AB_2338871 | ||
|
Rabbit Goat |
Iba1 Anti-rabbit IgG |
1:250 1:100 |
abcam/ab178846 Jackson Alexa 488/AB_2338046 | ||
|
Mouse Goat |
TLR4 Anti-mouse IgG |
1:100 1:100 |
abcam/ab22048 Jackson Alexa 594/AB_2338871 | ||
|
Mouse Goat |
GFAP Anti-mouse IgG |
1:100 1:100 |
CST/#3670 Jackson Alexa 594/AB_2338871 | ||
|
Rabbit Goat |
NF-κB (p65) Anti-rabbit IgG |
1:100 1:100 |
abcam/ab16502 Jackson Alexa 488/AB_2338046 | ||
|
Rabbit Goat |
GFAP Anti-rabbit IgG |
1:100 1:100 |
abcam/ab104139 Jackson Alexa 488/AB_2338046 |