Skip to main content

Table 1 Multiple reaction monitoring transitions and optimized mass parameters for the analytes

From: UPLC-MS/MS method for simultaneously determining nucleosides and methyl-nucleosides in liver mRNA of Epimedin C-induced liver injury mouse model

Analytes Precursor ion (m/z) Product ion (m/z) DP (V) EP (V) CE (V) CXP (V)
Adenosine (A) 268.1 136.1 30 10 22 10
Uridine (U) 245.0 113.1 12 10 12 10
Cytidine (C) 244.1 112.0 30 10 13 10
Guanosine (G) 284.2 152.1 30 10 15 10
N6-Methyladenosine (m6A) 282.2 150.2 30 10 24 10
N5-Methylcytidine (m5C) 258.2 126.1 30 10 15 10
Lamivudine (IS) 230.2 112.0 30 10 14 10
  1. DP declustering potential, EP entrance potential, CE collision energy, CXP collision cell exit potential