Table 1 Multiple reaction monitoring transitions and optimized mass parameters for the analytes
Analytes | Precursor ion (m/z) | Product ion (m/z) | DP (V) | EP (V) | CE (V) | CXP (V) |
|---|---|---|---|---|---|---|
Adenosine (A) | 268.1 | 136.1 | 30 | 10 | 22 | 10 |
Uridine (U) | 245.0 | 113.1 | 12 | 10 | 12 | 10 |
Cytidine (C) | 244.1 | 112.0 | 30 | 10 | 13 | 10 |
Guanosine (G) | 284.2 | 152.1 | 30 | 10 | 15 | 10 |
N6-Methyladenosine (m6A) | 282.2 | 150.2 | 30 | 10 | 24 | 10 |
N5-Methylcytidine (m5C) | 258.2 | 126.1 | 30 | 10 | 15 | 10 |
Lamivudine (IS) | 230.2 | 112.0 | 30 | 10 | 14 | 10 |