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Table 2 Precision and accuracy data for the quantification of six nucleosides in mRNA (n = 5)

From: UPLC-MS/MS method for simultaneously determining nucleosides and methyl-nucleosides in liver mRNA of Epimedin C-induced liver injury mouse model

Analytes Concentration levels (mean ± SD, pg/mL) RSD (%) RE (%)
Added Measured Intra-day Inter-day Accuracy
Adenosine (A) 40 40.8 ± 1.9 6.5 4.3 2.0
120 133.3 ± 3.1 3.9 2.0 11.1
1600 1792.7 ± 33.7 3.6 1.4 12.0
16,000 15,120.0 ± 270.4 4.0 1.0 − 5.5
Uridine (U) 2000 1919.3 ± 96.0 6.5 4.7 − 4.0
6000 6101.3 ± 220.7 7.4 2.5 1.7
80,000 83,680.0 ± 1235.9 2.6 1.2 4.6
800,000 710,133.3 ± 10,405.1 2.1 1.3 − 11.2
Cytidine (C) 40 36.7 ± 2.3 0.5 6.8 − 8.3
120 117.3 ± 5.4 4.8 4.5 − 2.3
1600 1672.7 ± 26.0 1.5 1.6 4.5
16,000 15,953.3 ± 247.5 1.2 1.6 − 0.3
Guanosine (G) 200 195.0 ± 12.0 12.7 4.1 − 2.5
600 630.4 ± 21.8 6.4 2.7 5.1
8000 8472.0 ± 280.3 4.3 3.1 5.9
80,000 78,180.0 ± 1894.1 4.5 1.9 − 2.3
N6-Methyladenosine (m6A) 40 36.2 ± 2.0 13.8 1.9 − 9.5
120 131.2 ± 6.9 2.4 5.6 9.3
1600 1733.3 ± 96.0 7.2 5.2 8.3
16,000 15,806.1 ± 196.9 1.2 1.2 − 1.2
N5-Methylcytidine (m5C) 40 38.4 ± 1.6 2.1 4.3 − 4.0
120 122.3 ± 3.3 4.2 2.3 1.9
1600 1697.3 ± 39.9 6.0 0.6 6.1
16,000 15,726.7 ± 286.5 3.3 1.4 − 1.7