Table 4 Precision and accuracy of the dilution QC samples (n = 5)
Analytes | Added (ng/mL) | Dilution factor | Caculated (pg/mL) | Measured (pg/mL) | RSD (%) | RE (%) |
|---|---|---|---|---|---|---|
Adenosine | 12 | 100 | 120 | 137.8 ± 2.2 | 1.6 | 14.8 |
1600 | 100 | 16,000 | 14,940.0 ± 167.3 | 1.1 | − 6.6 | |
Uridine | 600 | 100 | 6000 | 5862.0 ± 99.3 | 1.7 | − 2.3 |
80,000 | 100 | 800,000 | 716,000.0 ± 10,583.0 | 1.5 | − 10.5 | |
Cytidine | 12 | 100 | 120 | 115.8 ± 5.4 | 4.6 | − 3.5 |
1600 | 100 | 16,000 | 16,100.0 ± 339.1 | 2.1 | 0.6 | |
Guanosine | 60 | 100 | 600 | 635.2 ± 26.0 | 4.1 | 5.9 |
8000 | 100 | 80,000 | 78,920.0 ± 1856.6 | 2.4 | − 1.4 | |
m6A | 12 | 100 | 120 | 126.4 ± 10.2 | 8.0 | 5.3 |
1600 | 100 | 16,000 | 15,631.2 ± 212.5 | 1.4 | − 2.3 | |
m5C | 12 | 100 | 120 | 125.8 ± 4.5 | 3.6 | 4.8 |
1600 | 100 | 16,000 | 16,540.0 ± 320.9 | 1.9 | 3.4 |