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Table 4 Precision and accuracy of the dilution QC samples (n = 5)

From: UPLC-MS/MS method for simultaneously determining nucleosides and methyl-nucleosides in liver mRNA of Epimedin C-induced liver injury mouse model

Analytes Added (ng/mL) Dilution factor Caculated (pg/mL) Measured (pg/mL) RSD (%) RE (%)
Adenosine 12 100 120 137.8 ± 2.2 1.6 14.8
1600 100 16,000 14,940.0 ± 167.3 1.1 − 6.6
Uridine 600 100 6000 5862.0 ± 99.3 1.7 − 2.3
80,000 100 800,000 716,000.0 ± 10,583.0 1.5 − 10.5
Cytidine 12 100 120 115.8 ± 5.4 4.6 − 3.5
1600 100 16,000 16,100.0 ± 339.1 2.1 0.6
Guanosine 60 100 600 635.2 ± 26.0 4.1 5.9
8000 100 80,000 78,920.0 ± 1856.6 2.4 − 1.4
m6A 12 100 120 126.4 ± 10.2 8.0 5.3
1600 100 16,000 15,631.2 ± 212.5 1.4 − 2.3
m5C 12 100 120 125.8 ± 4.5 3.6 4.8
1600 100 16,000 16,540.0 ± 320.9 1.9 3.4