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Fig. 2 | Chinese Medicine

Fig. 2

From: Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Fig. 2

Nuclear morphology and cell cycle distribution of H22 cells upon MPEE treatment. H22 cells were treated with different concentrations of MPEE for 24 h. A After staining with Hoechst 33258, nuclear morphology of H22 cells was observed by inverted fluorescence microscopy. The arrows indicated the chromosomal condensation. BC Cell cycle phase distribution was analyzed by flow cytometry following PI staining. D Heatmap of clustered cell cycle associated genes as evaluated by transcriptome analysis. E The mRNA levels of Cdk2, Cyclin D1, Gadd45α, Cdk1, Mcm2, Mcm4, Cyclin B1 and Cdc25b were analyzed by qRT-PCR. F The protein levels of Cyclin B1, Cyclin D1 and Cdk2 were detected by Western blot. Data were analyzed by ANOVA. **p < 0.01; ***p < 0.001 compared to untreated group

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