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Fig. 4 | Chinese Medicine

Fig. 4

From: Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Fig. 4

The effects of MPEE on Δψm and caspase cascade in H22 cells. H22 cells were treated with different concentrations of MPEE for 24 h. A, B Cells were stained with JC-1 and the fluorescence changes were analyzed by flow cytometry. C The protein levels of Bax, Bcl-2 and cytochrome c were detected by Western blot. D The mRNA levels of Bax and Bcl-2 were analyzed by qRT-PCR. E The levels of cleaved-caspases and -PARP were detected by Western blot. Data were analyzed by ANOVA. ***p < 0.001 compared to untreated group

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