Fig. 3
Increased G2/M ratio with alternative expression of indicated proteins by EVO in SW1736 and KAT4B cells. A Increased G2/M ratio by EVO in SW1736 (Upper panel) and KAT4B (Lower panel) cells. Both cell lines were treated with and without EVO (8 µM) for different times (6, 12, 24, and 30 h), and cell cycle progression was analyzed by flow cytometry via PI staining. A representative of the flow cytometric analysis was shown on the top of each panel; data were calculated from three triplicate groups and statistically analysis was shown below. B Alternative expressions of indicated proteins including p53, phosphorylated (p)-p53, cdc25c, cdc2, p27, and p21 proteins in SW1736 (left panel) and KAT3B cells (right panel) under EVO (8 µM) stimulation for different times (6, 12, and 24 h). Both cell lines were treated with EVO for different times, and expressions of indicated proteins were examined by Western blotting using specific antibodies. The intensity of each band was examined by a densitometric analysis (Image J), and results are expressed as multiples of the control. Each data point was calculated from triplicate groups, and data are shown as the mean ± SD. *p < 0.05 and **p < 0.01 denote a significant difference between indicated groups