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Fig. 5 | Chinese Medicine

Fig. 5

From: Roles of reactive oxygen species, mitochondrial membrane potential, and p53 in evodiamine-induced apoptosis and G2/M arrest of human anaplastic thyroid carcinoma cells

Fig. 5

Structure-activity relationship of EVO and related chemicals on viability, DNA ladders, MMP, and ROS production in SW1736 cells. A The chemical structures of EVO and structurally related chemicals (EVO, -4, -5, -8, -9, and -12) are depicted. B Differential apoptotic effects elicited by EVOs in SW1736 cells. C Differential cytotoxicity of EVOs on the viability of SW1736 cells. D Differential morphological changes by EVOs in SW1736 cells. SW1736 cells were treated with the indicated EVOs (8 µM) for 12 h, and DNA integrity, viability, and morphology of SW1736 cells were analyzed by agarose electrophoresis, MTT assay, and Giemsa staining, respectively. Scale bar (), 100 μm. E Effects of EVOs on MMP and intraceullular peroxide production in SW1736 cells. SW1736 cells were treated with indicated EVOs for 12 h followed by detecting MMP and peroxide levels via DiOC6 and DCFH-DA staining via flow cytometric analysis. (F) IC50 values of EVO, EVO-4, -8, -12 on viability of SW1736 cells were examined by MTT assay. SW1736 were treated with different concentrations (2, 4, 8, and 16 µM) for 12 h, and viability of cells was measured by MTT assay. (Top) a representative of MTT assay; (Lower) IC50 values were measured via calculating the % cell viability and using equation by putting the 50 in the Y-value. Each data point was calculated from three triplicate groups, and data are displayed as the mean ± S.D. **p < 0.01 denotes a significant difference compared to the control (CON)

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