Fig. 6

Differential actions of microtubule disruptors including EVO, paclitaxel (TAX), and nocodazole (NOC) on the ratio of hypodiploid cells, and ratio of cells in G1, S, and G2/M phase in ATC cells SW1736 (p53+/+) and KAT4B (p53 mut/mut) cells. SW1736 (Upper panel) and KAT-4B (Lower panel) cells were treated with indicated concentrations of EVO, TAX, and NOC for 24 h, and ratio of cells at sub-G1 (hypodiploid cells), G1, S, and G2/M phase was measured by flow cytometry via PI staining. A representative of the flow cytometric analysis was shown on the top of each panel; data were calculated from three triplicate groups and statistically analysis was shown below. Each data point was calculated from triplicate groups, and data are shown as the mean ± SD. *p < 0.05, **p < 0.01 denotes a significant difference from control group (C)