Fig. 3

SKN inhibits expression levels of pro-inflammatory mediators in CIA mice. From the day of intensive immunization, CIA mice were given 4 mg/kg SKN and 0.5 mg/kg MTX for 20 consecutive days. The expression of TNF-α (A,B,G), IL-1β(C,D,H), IL-6 (E,F,I) in synovium and serum of knee joints of mice. The experiments were carried out in triplicate indenpendently and calculated Mean ± SEM (n = 6). ***P < 0.001, **P < 0.01, and *P < 0.05, compared with the CIA group; ^##P < 0.01, ^###P < 0.001, compared with the control group