Fig. 7

SKN targeted on SOCS1 in TNF-α-induced FLS and CIA mice. A, D FLS which seeded in 48-well plates in the presence of 20 ng/ml TNF-α with or without SKN (0.05, 0.1 and 0.2 μM) for 24 h and tested by IF. The number of vimentin + SOCS1 + cells were counted. B, C The number of vimentin + SOCS1 + cells was less in the joints of CIA mice of CIA group, while it was more in SKN group. At the same time, we silenced the SOCS1 gene in FLS. PCR results showed that shikonin could significantly increase the expression of SOCS1 in FLS silencing SOCS1 (Fig. 7E). All these indicate that shikonin acts on SOC in synovial cells. The experiments were carried out in triplicate indenpendently and calculated Mean ± SEM (n = 3 in vitro or 6 in vivo). *P < 0.05, ** P < 0.01, *** P < 0.001, compared with the TNF-α-induced /CIA group group; ^###P < 0.001, compared to the control group. ***P < 0.001, **P < 0.01, and *P < 0.05, compared with the TNF-α-induced /CIA group; ^##P < 0.01, ^###P < 0.001, compared with the control group