Fig. 6

Visualization of MAPK3 expressed in ovarian granulosa cells of the PCOS, BSTJF and control groups by double-labeling immunofluorescence with FoxL2 (winged helix for head transcription factor gene2, specific expression in adult ovarian granulosa cells) (green) and MAPK3 (red). Nuclei were counterstained with 40,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). All scale bars are 100 μm. The expression ratio of the target protein is shown by bar plots (n = 4 in each group). Data were presented as the mean ± SEM. One-way ANOVA for multiple comparisons and LSD test for pairwise comparisons between groups