Fig. 7

Neurobehavioral and cerebral morphological changes in the female offspring. A Morris water maze test in the PCOS female offspring (n = 4 in 5 weeks, n = 4 in 13 weeks), BSTJF (n = 4 in 5 weeks, n = 4 in 13 weeks) and controls (n = 4 in 5 weeks, n = 4 in 13 weeks). Data are presented as the mean ± SEM. One-way ANOVA for multiple comparisons and LSD test for pairwise comparisons between groups (*P < 0.05, ^#P < 0.01). B The morphological manifestation of neurons in the dentate gyrus of female offspring by Nissl staining. The scale bars are 100 μm. C The morphological structure of the dendrites in the dentate gyrus of female offspring by Golgi staining. The scale bars are 40 μm. Representative dendrites are partially enlarged, and the dendritic spines are indicated in blue. The scale bars are 20 μm. The density of dendritic spines is shown by bar plots (n = 4 in each group at 5 w and 13 w). Data are presented as the mean ± SEM, one-way ANOVA for multiple comparisons and LSD test for pairwise comparisons between groups (*P < 0.05, ^#P < 0.01)