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Table 1 The HPLC method validation for simultaneous quantification of the four markers in UTE and URE

From: Comparison of the chemical constituents and anti-Alzheimer’s disease effects of Uncaria rhynchophylla and Uncaria tomentosa

Markers

Regression equation

Linear ranges (μg/mL)

r2

LOD (μg/mL)

LOQ (μg/mL)

Precision (RSD%)

Repeatability (RSD%)

Stability (RSD%)

Recovery (UTE)

Recovery (URE)

UTE

URE

UTE

URE

Mean (%)

RSD (%)

Mean (%)

RSD (%)

Rhynchophylline

y = 211.5x − 252.6

10–1000

0.9998

1.91

9.15

2.77

2.79

1.75

2.05

1.14

100.98

1.50

97.98

1.73

Isorhynchophylline

y = 223.7x − 2403

10–1000

0.9995

2.09

10.26

1.62

1.95

2.31

1.32

2.15

99.27

1.35

99.30

0.57

Corynoxeine

y = 152.6x + 1399

10–1000

0.9997

1.83

9.07

1.83

2.46

2.71

2.09

1.62

100.63

1.45

102.84

0.73

Isocorynoxeine

y = 107.7x − 140.4

10–500

0.9999

2.03

10.22

2.54

1.80

2.57

1.05

2.04

101.62

1.46

103.19

1.30

  1. x, concentration in μg/mL; y, peak area; r2, correlation coefficient of regression equations
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Chinese Medicine

ISSN: 1749-8546

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