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Fig. 8 | Chinese Medicine

Fig. 8

From: NMPA-approved traditional Chinese medicine-Pingwei Pill: new indication for colistin recovery against MCR-positive bacteria infection

Fig. 8

Pingwei Pill combined with colistin effectively protected chicks from Salmonella sp. strain HYM2 infection. A Scheme of the experimental protocols for the chick infection models. Streptomycin-treated one-day-old chicks were infected with Salmonella sp. strain HYM2 (1.0 × 109 CFUs/chick for survival analysis and 1.0 × 107 CFUs/chick for other analyses) and treated with colistin (5 mg/kg), Pingwei Pill (0.8 g/kg), a combination of colistin (5 mg/kg) plus Pingwei Pill (0.8 g/kg), or PBS. The survival (B, n = 10) and body weight (C, n = 5) of infected chicks with the indicated treatment at 168 h post infection. At 3 days post infection, chicks (n = 10) in each group were sacrificed, and the cecum was taken for the determination of gross pathological analysis (D) and histopathological analysis using hematoxylin and eosin (H&E) (E). Following homogenization, the bacterial burden was determined by plating serial dilutions of the supernatants on agar plates (F), and TNF-α in the supernatants was examined using ELISAs (G). All the data were expressed as means ± S.D. (n ≥ 5). **P < 0.01

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