Integrated analysis of the chemical-material basis and molecular mechanisms for the classic herbal formula of Lily Bulb and Rehmannia Decoction in alleviating depression

Table 6 The differently expressed mRNAs in CORT-induced cell depression model treated with LBRD standard decoction were regulated by miRNAs

miRNAs	The predicted target mRNAs that match DEGs in transcriptome^a
rno-miR-7a-2-3p ↓	Mbp ↑ Bdnf ↑ Calml4 ↑
rno-miR-532-3p ↓	Mbp ↑ Mefv ↑ Psd3 ↑ Npas2↑
rno-miR-495 ↓	Gad1↑ Bdnf ↑ Pex12 ↑ Wipf3 ↑ Calml3 ↑ S1pr3 ↑ Psd3 ↑ Calml4 ↑ Npas2 ↑
rno-miR-336-5p ↓	Bdnf ↑ Mefv ↑ Psd3 ↑ Calml4↑
rno-miR-24-3p ↓	Nr1d1↑
rno-miR-23a-5p ↓	Ntrk2 ↑ VGAT ↑ Psd3 ↑
rno-miR-144-3p ↓	Gad1↑ Ntrk2 ↑ Mefv ↑ VGAT ↑ Wipf3 ↑ Psd3 ↑ Calml4↑
rno-miR-130b-5p ↓	Mbp ↑ Gad1↑ Bdnf ↑ Ntrk2 ↑ Enpep ↑ Wipf3 ↑ Calml4 ↑ Npas2↑
rno-miR-125b-5p ↓	Mbp ↑ Bdnf ↑ Ntrk2 ↑ Enpep ↑ Wipf3 ↑ Itgb6 ↑ S1pr3 ↑ Calml4 ↑
rno-let-7b-3p ↑	Zswim5 ↓ Lifr↓
rno-miR-708-5p ↑	Zswim5 ↓ Crb3 ↓ Lifr↓
rno-miR-34c-5p ↑	Ghrhr↓
rno-miR-331-3p ↑	Crb3↓

^aThe target mRNAs predicted by TargetScan, Rnahybrid and Miranda, and then overlapped to DEGs in transcriptome. ↓, miRNA significantly down-regulated in LBRD standard decoction-containing serum group vs CORT group, and CORT group vs vehicle group; ↑, up-regulation

ISSN: 1749-8546