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Fig. 1 | Chinese Medicine

Fig. 1

From: Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma

Fig. 1

Aloin inhibited osteosarcoma cell proliferation in vitro. A Human osteosarcoma cell lines (HOS & MG63) were treated with different concentrations of aloin for 24, 48 or 72 h before MTT assays were performed. B HOS & MG63 cells were treated with different concentrations of aloin (0, 100 and 300 μM) for 24 h before Hoechst 33,342 staining was utilized for apoptosis morphology analysis. C HOS & MG63 cell lines were incubated with aloin for 24 h before the FITC-annexin V/PI apoptosis detection kit was utilized for flow cytometry. Error bar = mean ± SD of at least triplicate experiments. (* P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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