Fig. 11
From: Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma
Histological detection in organs and tumors. A IHC and HE staining were utilized to assess tumor tissue sections. The markers examined were LC3B, mTOR, pho-mTOR, PI3Kα, AKT, and pho-AKT. B Toxic effects on organs were tested by HE staining. C The expression levels of each marker were quantified by ImageJ (v1.8). Magnification, × 200 (enlarged pictures), × 40 (unenlarged pictures). Error bar = mean ± SD of at least triplicate experiments. (* P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001)