Fig. 5
From: Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma
Autophagy assay in aloin-treated HOS & MG63 cells. A Transmission electron microscopy scanning of aloin-treated HOS & MG63 cells to observe autophagosome formation. B Dual-fluorescence autophagy flux assay in aloin-treated HOS & MG63 cells. The wavelength was 488 nm and 560 nm. A fluorescence spot count was applied by ImageJ (v1.8). Error bar = mean ± SD of at least triplicate experiments. (* P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001)