Fig. 6
From: Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma
Aloin and chloroquine coadministration for turnover assays in HOS and MG63 cell lines. A After 24 h of treatment, MTT assays (A), flow cytometry assays (B), and dual fluorescence autophagy flux assays were carried out. Dual fluorescence spot quantitative analysis was performed by ImageJ (v1.8). Flowcyto clustering was applied by FLOWJO 10. Error bar = mean ± SD of at least triplicate experiments. (* P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001)