Skip to main content
Fig. 5 | Chinese Medicine

Fig. 5

From: An alcohol extract prepared from the male flower of Eucommia ulmoides Oliv. promotes synoviocyte apoptosis and ameliorates bone destruction in rheumatoid arthritis

Fig. 5

The apoptosis-inducing effects of EF in TNF-α-stimulated HFLS-RA cells. HFLS-RA cells were incubated with TNF-α (10 ng/mL) and EF (400, 800, 1600 µg/mL) for 24 h. A Percent apoptosis was determined by flow cytometry. Apoptotic cells were detected by staining with Annexin V-FITC/PI followed by flow cytometry. B Data are the mean ± SD (n = 4), *p < 0.05, **p < 0.01, ***p < 0.001, vs. TNF-α cells (HFLS-RA cells treated by TNF-α alone). #p < 0.05, ##p < 0.01, ###>p < 0.001, vs. control cells. C mRNA expression of IL-1β, IL-6, VEGF, MMP-9, caspase-3, Bax, and Bcl-2 was determined by real-time RT-qPCR. Data are the mean ± SD (n = 4), *p < 0.05, **p < 0.01, ***p < 0.001, vs. TNF-α cells (HFLS-RA cells treated by TNF-α alone). #p < 0.05, ##p < 0.01, ###p < 0.001, vs. control cells

Back to article page