Fig. 7

EF inhibited differentiation into osteoclasts in RANKL-stimulated RAW264.7 cells via the NF-κB pathway. A Toxicity of EF in cultured cells was tested for 24 h. B RAW264.7 cells were incubated with LPS (1 µg/mL) and EF (100, 200, 400, 800, 1600, 2000 µg/mL) for 24 h. C TRAP staining was used to investigate the effect of EF on the differentiation of RAW264.7 cells into osteoclasts. D Western blotting was conducted to determine the protein expression of p-p65 NF-κB, p-Iκκαβ, p-IκBα, p65 NF-κB, Iκκαβ, and IκBα. E Data are the mean ± SD (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001, vs. TNF-α cells. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, vs. control cells