Table 1 Sequences of the primers used for RT-qPCR to determine the mRNA expression of proinflammatory factors in HFLS-RA cells
Gene | 5′–3′ | Sequence |
|---|---|---|
GAPDH | Forward | CATGAGAAGTATGACAACAGCCT |
Reverse | AGTCCTTCCACGATACCAAAGT | |
IL-1β | Forward | TTCGACACATGGGATAACGAGG |
Reverse | TTTTTGCTGTGAGTCCCGGAG | |
IL-6 | Forward | ACTCACCTCTTCAGAACGAATTG |
Reverse | CCATCTTTGGAAGGTTCAGGTTG | |
MMP-9 | Forward | TGTACCGCTATGGTTACACTCG |
Reverse | GGCAGGGACAGTTGCTTCT | |
VEGF | Forward | AGGGCAGAATCATCACGAAGT |
Reverse | AGGGTCTCGATTGGATGGCA | |
Caspase3 (cleaved) | Forward | TTACAGTGAACACCTCTACCAATGCCCCA |
Reverse | TCCGTGAAAACACTAATCACCTTGCCCCA | |
Bax | Forward | GGGAGACACCTGAGCTGACC |
Reverse | GGACTCCAGCCACAAAGATGG | |
Bcl-2 | Forward | GAACTGGGGGAGGATTGTGGCC |
Reverse | TCGACGTTTTGCCTGAAGACTGTTAA |