Fig. 2
Effects of SWT on CCl4-induced fibrotic injury in mice. CCl4-induced fibrotic liver injury in mice was established as described in the method. Different doses of SWT were administrated for 3 weeks as indicated. A Schematic diagram of in vivo experiments that linked SWT with anti-fibrotic therapies. B Ratio of liver and spleen to body weight. C ALT, AST and ALP levels in serum. D Hepatic hydroxyproline levels. E Representative images of H&E, Masson’s Trichrome staining and immunohistochemistry staining against FN of liver tissues. Scale bar = 100 μm. F Relative mRNA levels of Tgfb1, H19, Acta2, Col1a1 and Fn1 in liver tissues were determined by qPCR and normalized using Hprt1 as an internal control. G Representative immunoblots against α-SMA, COL1, FN and β-ACTIN in liver tissues were shown. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, compared with control group; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with CCl4 group. One-way ANOVA with Tukey’s post-hoc tests (n = 8)