Fig. 7
Effects of SWT on FXR and its downstream signaling in mice with fibrotic liver injury. Relative mRNA levels of A Slc10a2, Abcc2, Osta, Ostb, C Nr1h4 (left panel) and D Fgf15 (left panel) in the intestine, B Abcb11, Slc10a1, Osta, Ostb, D Fgfr4 (right panel), E Nr1h4 (left panel), F Nr0b2 and Cyp7a1 in the liver were determined by qPCR and normalized using Hprt1 as an internal control. Representative immunoblots against FXR and β-ACTIN in the intestine (C, right panel) and liver (E, right panel) were shown. D Serum levels of FGF15 were determined by FGF15 ELISA assay kit (middle panel). G The role of SWT in regulating “liver-gut axis” involved pathways. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, compared with control group; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with CCl4 group. One-way ANOVA with Tukey’s post-hoc tests (n = 8)