Fig. 3
From: Curcumin prevents As3+-induced carcinogenesis through regulation of GSK3β/Nrf2
Curcumin alleviated As3+-induced oxidative stress by activating Nrf2. BEAS-2B cells were treated with 3.125, 6.25, 12.5 and 25 μM curcumin and/or 10 μM As3+ for 12 h. ROS levels were detected by DCF staining and detected by microscope and flow cytometry. A Normal BEAS-2B cells were treated with 3.125, 6.25 and 12.5 μM curcumin and/or 10 μM As3+ for 24 h. The treatment cells were harvested and whole protein lysates were extracted. Nrf2, HO-1, SOD-2 and Bcl-2 expression was examined by immunoblotting. B Normal BEAS-2B cells were transfected with either control-siRNA or Nrf2-siRNA for 48 h. BEAS-2B cells were then treated with 3.125, 6.25 and 12.5 μM curcumin and/or 10 μM As3+ for 24 h. The treatment cells were harvested and whole protein lysates were extracted. Nrf2, HO-1, SOD-2 and Bcl-2 expression was examined by immunoblotting. C DCF-based fluorescence was measured using flow cytometry. Cell lysates were subjected to immunoblot analysis. The data are expressed as the mean ± SD of three independent experiments (n = 9). *p < 0.05, **p < 0.01 versus normal BEAS-2B control group