Fig. 4From: Curcumin prevents As3+-induced carcinogenesis through regulation of GSK3β/Nrf2Curcumin increased As3+ -induced autophagy in normal BEAS-2B cells. A The mCherry-GFP-LC3 reporter is red and green when it associates with phagophores and autophagosomes. After fusion with lysosomes to form autolysosomes, the GFP signal is quenched by the acidic condition. The normal BEAS-2B (A) transfected with the mCherry-EGFP-LC3 construct and treated with As3+ and/or curcumin for 24 h. White arrows indicate autophagosome, yellow arrows indicate autolysosome. B Normal BEAS-2B cells were treated with 6.25, 12.5 μM curcumin and/or 10 μM As3+ for 24 h. The treatment cells were harvested and whole protein lysates were extracted. LAMP1, TFEB and LC-3II expression was examined by immunoblotting. The data are expressed as the mean ± SD of three independent experiments (n = 9). *p < 0.05, **p < 0.01 versus normal BEAS-2B control groupBack to article page