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Fig. 9 | Chinese Medicine

Fig. 9

From: Curcumin prevents As3+-induced carcinogenesis through regulation of GSK3β/Nrf2

Fig. 9

Curcumin suppressed angiogenesis through modulating Nrf2-mediated HIF1α/ VEGF signaling pathway in AsT cells. A AsT cells and their parent non-transformed BEAS-2B cells were exposed to increasing concentrations (3.125, 6.25, 12.5 μM) of curcumin for 24 h, the levels of Nrf2, HIF1α and VEGF were detected by western blotting. B AsT cells and their parent non-transformed BEAS-2B cells were exposed to increasing concentrations (6.25, 12.5, 25 μM) of curcumin for 24, 48 and 72 h. Cell proliferations were detected by MTT assay. C AsT cells were transfected with control siRNA or Nrf2 siRNA for 24 h prior to 25 μM curcumin treatment for 24 h. the levels of Nrf2, HIF1α and VEGF were detected by western blotting and D Cell proliferations of 24, 48 and 72 h curcumin (25 μM) treatment were detected by MTT assay. AsT cells were treated with 5 μM SB216763 and/or 25 μM curcumin for 24 h, E the levels of p-GSK3β s9, GSK3β, Nrf2, HIF1α, VEGF and MMP2/9 were detected by western blotting. F Cell viabilities were detected by staining with MTT assay. G Cell invasion were detected by Transwell Migration Assay. HUVECs were incubated in 5 μM SB216763 and/or 25 μM curcumin treated AsT cells culture medium and tube formation were detected by Matrigel assay. The quantitative analysis of migration (H) and tube formation (I). The data are expressed as the mean ± SD of three independent experiments (n = 9). *p < 0.05 versus normal BEAS-2B control group; &p < 0.05, &&p < 0.01 versus AsT cells control group

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