Fig. 4

HK attenuates lipid accumulation by restoring mitochondrial function. AML12 cells were treated with or without HK in the presence of P/O for 24 h. A The relative Rhod123 fluorescent intensity. B Intracellular ROS accumulation levels were determined by DCFH-DA assay. C Mitochondria were stained by Mitochondria-green and quantitated by flow cytometry. D Relative glycerol content in P/O-stimulated AML12 hepatocytes under both normal and isoproterenol-induced conditions. E The acetylated and total LCAD levels. Data was expressed as means ± SD. **p < 0.01 and ***p < 0.001, HK vs. P/O treatment. ##p < 0.01 and ###p < 0.001 ctrl vs. P/O treatment. One-way ANOVA was used to calculate the p-values