Fig. 9

a Immunofluorescent staining of TUNEL (× 400) in CON, COH and BAL group. Apoptotic cells are stained by green color, and the nucleus is stained by blue. The apoptosis-positive staining assembled in the GCL (arrow). Scale bar = 20 μm. b The apoptosis rate in GCL (TUNEL/DAPI) comparison among these three groups. c Immunofluorescent staining expression of BCL-2 (× 400) in CON, COH and BAL group. Scale bar = 50 μm. d Quantitative analysis of BCL-2 fluorescent positive protein expression. All data are shown as mean ± SD, n = 3. *P < 0.05, **P < 0.01, ****P < 0.0001