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Fig. 1 | Chinese Medicine

Fig. 1

From: Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo

Fig. 1

Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL (C-D) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F, G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I, J The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared with the control group, #P < 0.05, ##P < 0.01 compared with LPS + ATP group

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