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Fig. 3 | Chinese Medicine

Fig. 3

From: Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo

Fig. 3

The antioxidant effect of HKL in BEAS-2B cells depends on the activation of Nrf2. After transfection with negative control siRNA (siNC) or Nrf2 siRNA (siNrf2) for 6 h, the cell medium was changed and BEAS-2B cells continued to be cultivated for up to 48 h. At 24 h before harvest, the cells were treated with HKL (25 μM) for 20 h, and then stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min. A Flow diagrams of cell experiments. B, C The protein expression of Total-Nrf2, Nul-Nrf2 and HO-1 and D mRNA expression of Nrf2 and HO-1 were analyzed by western blot and RT-PCR. E–G The levels of MPO, MDA and SOD in supernatant were quantified by ELISA. Values are expressed as the mean ± SD of three independent experiments. **P < 0.01 compared with the siNC group; #P < 0.05, ##P < 0.01 compared with siNC + LPS + ATP group; &P < 0.05, &&P < 0.01 compared with siNrf2 group

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