Fig. 5

Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis through activation of Nrf2 in BEAS-2B cells. A, B Hoechst 33,342 /PI double-fluorescent staining was used to assess the pyroptosis of LPS and ATP-stimulated BEAS-2B cells (original magnification × 100). C The content of LDH in supernatant was measured by an LDH assay kit. D, E The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N and (F) mRNA expression of NLRP3, ASC, CASP1, GSDMD were analyzed by western blot and RT-PCR. G, H The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ^**P < 0.01 compared with the siNC group; ^#P < 0.05, ^##P < 0.01 compared with siNC + LPS + ATP group; ^&P < 0.05, ^&&P < 0.01 compared with siNrf2 group