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Fig. 8 | Chinese Medicine

Fig. 8

From: Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo

Fig. 8

Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis in rats with ALI via activation of Nrf2. A–B, D–E Relative expression levels of NLRP3, ASC, CASP1-P20, GSDMD-N protein in lung tissues were analyzed by western blot. C, F Relative expression levels of NLRP3, ASC, CASP1, GSDMD mRNA in lung tissues were analyzed by RT-PCR. G–I The content of IL-18, IL-1β and LDH in bronchoalveolar lavage fluid of each group were detected by ELISA. J Immunohistochemical staining of GSDMD (original magnification 400 ×) in the rat lungs. K The average optical density (AOD) was used to evaluate the intensity of IHC staining and measured by Image-Pro Plus 6.0 software. Data were analyzed using one-way ANOVA; n = 7–8 rats per group. **P < 0.01 compared with the control group; #P < 0.05, ##P < 0.01 compared with LPS group

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