Table 1 Sequences of primers for RT-PCR
Gene | Primer sequence | Rat (5' to 3') | Human (5' to 3') |
|---|---|---|---|
NLRP3 | Forward primer | TCTTTGCGGCTATGTACTATCT | GGACTATTTCCCCAAGATTG |
| Â | Reverse primer | TTCTAATAGGACCTTCACGT | ACTCCACCCGATGACAGTT |
ASC | Forward primer | ATCCTGGACGCTCTTGAAAACTT | CTCACCGCTAACGTGCTGC |
| Â | Reverse primer | GCTCCTGTATGCCCATGTCTCTA | CTTGGCTGCCGACTGAGGA |
CASP-1 | Forward primer | CGGGCAAGCCAGATGTTTAT | GGGCTCTGTTTTTATTGGAA |
| Â | Reverse primer | AACCACTCGGTCCAGGAAATG | ATCTGGCTGCTCAAATGAA |
GSDMD | Forward primer | CCAACATCTCAGGGCCCCAT | GGACAGGCAAAGATCGCAG |
| Â | Reverse primer | TGGCAAGTTTCTGCCCTGGA | CACTCAGCGAGTACACATTCATT |
Nrf2 | Forward primer | GGACCTAAAGCACAGCCAAC | TCCAGTCAGAAACCAGTGGAT |
Reverse primer | ATCTCTGGTCTGCTGCAGAG | GAATGTCTGCGCCAAAAGCTG | |
HO-1 | Forward primer | CTTACACACCAGCCACACAG | AAGACTGCGTTCCTGCTCAAC |
Reverse primer | ACTGAGTGTGAGGACCCATC | AAAGCCCTACAGCAACTGTCG | |
GAPDH | Forward primer | GACATGCCGCCTGGAGAAAC | ACAACTTTGGTATCGTGGAAGG |
| Â | Reverse primer | AGCCCAGGATGCCCTTTAGT | GCCATCACGCCACAGTTTC |