Fig. 1

TNBS-induced IBD mice presented comorbidities of visceral pain and depression and P2Y12 expression in the mPFC was upregulated in IBD mice. a Time course of mechanical threshold in response to von Frey filaments (n = 6–8 mice). b Visceral hyperalgesia was evaluated by colorectal dilatation (CRD) (n = 6–8 mice). c Sucrose preference test (SPT) was used to evaluated depression-like behaviors. Mice’s preference for sucrose was recorded as: sucrose consumption(g) / (sucrose consumption(g) + water consumption(g)) × 100% (n = 6–8 mice). d Representative immunoblots of P2Y12 and β-actin protein expression in mPFC. e Densitometric analysis of P2Y12 protein normalized to the loading control (n = 3 mice). f P2Y12 are stained red and nuclei are stained blue with DAPI in the mPFC of IBD mice. Scale bars: 100 μm. The data are expressed as mean ± SEM. *p < 0.05, compared with the control group